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1. WO1997013838 - ISOLATION OF CELLULAR MATERIAL UNDER MICROSCOPIC VISUALIZATION

Publication Number WO/1997/013838
Publication Date 17.04.1997
International Application No. PCT/US1996/016517
International Filing Date 09.10.1996
Chapter 2 Demand Filed 09.05.1997
IPC
G01N 1/02 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
02Devices for withdrawing samples
G01N 1/04 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
02Devices for withdrawing samples
04in the solid state, e.g. by cutting
G01N 1/28 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
G01N 15/14 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation
G01N 33/50 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
CPC
C12M 33/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
33Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
02by impregnation, e.g. using swabs or loops
G01N 1/04
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
02Devices for withdrawing samples
04in the solid state, e.g. by cutting
G01N 1/08
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
02Devices for withdrawing samples
04in the solid state, e.g. by cutting
08involving an extracting tool, e.g. core bit
G01N 1/2806
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
2806Means for preparing replicas of specimens, e.g. for microscopal analysis
G01N 1/2813
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
2813Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
G01N 1/286
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
286involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
Applicants
  • THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES [US]/[US] (AllExceptUS)
  • LIOTTA, Lance, A. [US]/[US] (UsOnly)
  • EMMERT-BUCK, Michael [US]/[US] (UsOnly)
  • KRIZMAN, David, B. [US]/[US] (UsOnly)
  • CHUAQUI, Rodrigo [CL]/[US] (UsOnly)
  • LINEHAN, W., Marston [US]/[US] (UsOnly)
  • TRENT, Jeffrey, M. [US]/[US] (UsOnly)
  • BONNER, Robert, F. [US]/[US] (UsOnly)
  • GOLDSTEIN, Seth, R. [US]/[US] (UsOnly)
  • SMITH, Paul, D. [US]/[US] (UsOnly)
  • PETERSON, John, I. [US]/[US] (UsOnly)
  • POHIDA, Tom [US]/[US] (UsOnly)
Inventors
  • LIOTTA, Lance, A.
  • EMMERT-BUCK, Michael
  • KRIZMAN, David, B.
  • CHUAQUI, Rodrigo
  • LINEHAN, W., Marston
  • TRENT, Jeffrey, M.
  • BONNER, Robert, F.
  • GOLDSTEIN, Seth, R.
  • SMITH, Paul, D.
  • PETERSON, John, I.
  • POHIDA, Tom
Agents
  • WEBER, Kenneth, A.
Priority Data
08/544,38810.10.1995US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) ISOLATION OF CELLULAR MATERIAL UNDER MICROSCOPIC VISUALIZATION
(FR) ISOLATION D'UN MATERIAU SOUS VISUALISATION MICROSCOPIQUE
Abstract
(EN)
A method of microdissection which involves: forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes a selectively activatable adhesive layer which provides, for example, chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated the transfer surface and tissue sample are separated. During separation the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample and may be molecularly analyzed.
(FR)
L'invention porte sur un procédé de microdissection qui suit les étapes ci-après: création d'un champ d'image de cellules présentes sur l'échantillon de tissu au moyen d'un microscope; identification d'au moins une zone de cellules d'intérêt à partir du champ d'image de cellules, cette zone au moins de cellules d'intérêt comprenant des types de cellules différentes de zones de cellules contiguës; et extraction d'au moins ladite zone de cellules d'intérêt de l'échantillon de tissu. L'extraction est effectuée par contact de l'échantillon de tissu avec une surface de transfert qui peut être sélectivement activée de façon que des régions de ladite surface adhèrent à la zone de cellules d'intérêt à extraire. La surface de transfert comprend une couche adhésive sélectivement activable qui adhère, par exemple, chimiquement ou électrostatiquement aux zones sélectionnées de l'échantillon de tissu. Une fois activée, la surface d'adhérence est séparée de l'échantillon de tissu. Adhérant à la surface de transfert tout au long du processus de séparation, la zone de cellules d'intérêt est ainsi séparée de l'échantillon de tissu, puis est soumise à une analyse moléculaire.
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