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1. (WO1997012964) NUCLEIC ACID FRAGMENTS ENCODING STEREOSPECIFIC NITRILE HYDRATASE AND AMIDASE ENZYMES AND RECOMBINANT MICROORGANISMS EXPRESSING THOSE ENZYMES USEFUL FOR THE PRODUCTION OF CHIRAL AMIDES AND ACIDES
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1997/012964    International Application No.:    PCT/US1996/015969
Publication Date: 10.04.1997 International Filing Date: 03.10.1996
Chapter 2 Demand Filed:    17.04.1997    
IPC:
C12N 9/78 (2006.01), C12N 9/80 (2006.01), C12P 7/40 (2006.01), C12P 13/02 (2006.01), C12P 17/00 (2006.01), C12P 41/00 (2006.01)
Applicants: E.I. DU PONT DE NEMOURS AND COMPANY [US/US]; 1007 Market Street, Wilmington, DE 19898 (US) (AL, AM, AT, AU, AZ, BA, BB, BE, BF, BG, BJ, BR, BY, CA, CF, CG, CH, CI, CM, CN, CU, CZ, DE, DK, EE, ES, FI, FR, GA, GB, GE, GN, GR, HU, IE, IL, IS, IT, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MC, MD, MG, MK, ML, MN, MR, MW, MX, NE, NL, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SN, SZ, TD, TG, TJ, TM, TR, TT, UA, UG, UZ, VN only).
FALLON, Robert, Donald [US/US]; (US) (For US Only).
NELSON, Mark, James [US/US]; (US) (For US Only).
PAYNE, Mark, Scott [US/US]; (US) (For US Only)
Inventors: FALLON, Robert, Donald; (US).
NELSON, Mark, James; (US).
PAYNE, Mark, Scott; (US)
Agent: FLOYD, Linda, A.; E.I. du Pont de Nemours and Company, Legal Patent Record Center, 1007 Market Street, Wilmington, DE 19898 (US)
Priority Data:
60/004,914 06.10.1995 US
Title (EN) NUCLEIC ACID FRAGMENTS ENCODING STEREOSPECIFIC NITRILE HYDRATASE AND AMIDASE ENZYMES AND RECOMBINANT MICROORGANISMS EXPRESSING THOSE ENZYMES USEFUL FOR THE PRODUCTION OF CHIRAL AMIDES AND ACIDES
(FR) FRAGMENTS D'ACIDE NUCLEIQUE CODANT UNE NITRILE HYDRATASE STEREOSPECIFIQUE ET DES ENZYMES AMIDASES AINSI QUE DES MICROORGANISMES DE RECOMBINAISON EXPRIMANT CES ENZYMES UTILES POUR LA PRODUCTION D'AMIDES ET D'ACIDES CHIRAUX
Abstract: front page image
(EN)The present invention provides a nitrile hydratase nucleic acid fragment isolated from $i(Pseudomonas putida) which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitriles to the corresponding R- or S- amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjuction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.
(FR)Fragments d'acide nucléique de nitrile hydratase isolés à partir de $i(Pseudomonas putida), codant une activité nitrile hydratase capable de catalyser l'hydrolyse de certains nitriles racémiques vers les R- ou S- amides correspondants. L'invention porte également sur des micro-organismes transformés capable d'exprimer activement ladite activité nitrile hydratase. De plus, l'invention porte sur un transformant abritant le gène nitrile hydratase en conjonction avec un gène amidase, l'un et l'autre pouvant être co-exprimé et donner lieu respectivement à une nitrile hydratase active et à des enzymes amidases. L'invention porte enfin sur des méthodes de production de tels matériaux énantiomères.
Designated States: AL, AM, AU, AZ, BA, BB, BG, BR, BY, CA, CN, CU, CZ, EE, GE, HU, IL, IS, JP, KG, KP, KR, KZ, LC, LK, LR, LT, LV, MD, MG, MK, MN, MX, NO, NZ, PL, RO, RU, SG, SI, SK, TJ, TM, TR, TT, UA, US, UZ, VN.
African Regional Intellectual Property Organization (KE, LS, MW, SD, SZ, UG)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)