Search International and National Patent Collections

1. (WO1997008296) METHOD OF GROWING RICKETTSIAE IN IXODES SCAPULARIS TICK CELL CULTURE AND PREPARING ANTIGENS AND VACCINES OF RICKETTSIAE

Pub. No.:    WO/1997/008296    International Application No.:    PCT/US1996/013594
Publication Date: Mar 6, 1997 International Filing Date: Aug 23, 1996
IPC: A61K 39/02
C07K 14/29
Applicants: REGENTS OF THE UNIVERSITY OF MINNESOTA
OKLAHOMA STATE UNIVERSITY
Inventors: MUNDERLOH, Ulrike, G.
KURTTI, Timothy, J.
KOCAN, Katherine, M.
BLOUIN, Edmour, F.
EWING, Sidney, A.
Title: METHOD OF GROWING RICKETTSIAE IN IXODES SCAPULARIS TICK CELL CULTURE AND PREPARING ANTIGENS AND VACCINES OF RICKETTSIAE
Abstract:
The invention is directed to methods of culturing rickettsiae in Ixodes scapularis cell lines. The methods of the invention provide for culture of microorganisms such as Anaplasma marginale, Ehrlichia canis, and Rickettsia rickettsii. A method of the invention involves incubating a rickettsia with an Ixodes scapularis tick cell culture in a culture medium under reduced oxygen and increased CO2 at a sufficient temperature until growth of the rickettsia is detected. The culture medium comprises a medium suitable for the growth of invertebrate cells supplemented with an organic buffer. The cell culture method can be used in large scale production of rickettsia containing products useful in diagnostic assays and vaccine preparations.