Processing

Please wait...

PATENTSCOPE will be unavailable a few hours for maintenance reason on Saturday 31.10.2020 at 7:00 AM CET
Settings

Settings

Goto Application

1. WO1996042013 - MICROELECTROPHORESIS CHIP FOR MOVING AND SEPARATING NUCLEIC ACIDS AND OTHER CHARGED MOLECULES

Publication Number WO/1996/042013
Publication Date 27.12.1996
International Application No. PCT/US1996/010110
International Filing Date 07.06.1996
Chapter 2 Demand Filed 07.01.1997
IPC
G01N 27/447 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
CPC
G01N 27/44704
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
44704Details; Accessories
G01N 27/44773
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
44756Apparatus specially adapted therefor
44773Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
G01N 27/44791
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
44756Apparatus specially adapted therefor
44791Microapparatus
Applicants
  • VISIBLE GENETICS INC. [CA]/[CA] (AllExceptUS)
  • YAGER, Thomas, D. [US]/[CA] (UsOnly)
  • WATERHOUSE, Paul [CA]/[CA] (UsOnly)
  • IZMAILOV, Alexandre, M. [RU]/[CA] (UsOnly)
  • MARUZZO, Bruno [CA]/[CA] (UsOnly)
  • STEVENS, John, K. [US]/[CA] (UsOnly)
  • LARSON, Marina, T. [US]/[US] (UsOnly)
Inventors
  • YAGER, Thomas, D.
  • WATERHOUSE, Paul
  • IZMAILOV, Alexandre, M.
  • MARUZZO, Bruno
  • STEVENS, John, K.
  • LARSON, Marina, T.
Agents
  • LARSON, Marina, T.
Priority Data
60/000,03608.06.1995US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MICROELECTROPHORESIS CHIP FOR MOVING AND SEPARATING NUCLEIC ACIDS AND OTHER CHARGED MOLECULES
(FR) PUCE DE MICROELECTROPHORESE POUR DEPLACER ET SEPARER DES ACIDES NUCLEIQUES ET AUTRES MOLECULES CHARGEES
Abstract
(EN)
A microelectrophoresis chip comprises a substrate in which there are formed one or more channels, one channel for each sample to be evaluated. The channels extend for the length of the chip, a distance of generally around 1 cm, and are about 1 to 10 $g(m)m wide and 1 to 10 $g(m)m in depth. The channels are filled with a homogeneous separation matrix which acts as an obstacle to the electrophoretic migration of the charged molecules. Microelectrodes disposed in the channels are used to induce an electric field within the homogeneous separation medium. When a voltage is applied across two or more of the microelectrodes, the charged molecules are induced to move and separate according to the electric field density, the type of solvent film, and the charge, shape and size of the charged molecule. The chip may further comprise detectors, such as light polarization detectors, fluorescence emission detectors, biosensors, electrochemical sensors or other microcomponents which may include sites for enzymatic or chemical manipulation of the moved or separated charged molecules.
(FR)
La présente invention se rapporte à une puce de microélectrophorèse comportant un substrat dans lequel un ou plusieurs canaux sont formés, un canal pour chaque échantillon à évaluer. Les canaux s'étendent tout le long de la puce, c'est-à-dire une distance d'environ 1 cm en général, et ont une largeur d'environ 1 à 10$g(m)m et une profondeur d'environ 1 à 10$g(m)m. Les canaux sont remplis avec une matrice de séparation homogène agissant en tant qu'obstacle à la migration électrophorétique des molécules chargées. Les microélectrodes placées dans les canaux servent à induire un champ électrique à l'intérieur du milieu de séparation homogène. Lorsqu'une tension est appliquée entre deux ou plusieurs microélectrodes, les molécules chargées sont amenées à se déplacer et à se séparer selon la densité du champ électrique, le type de film de solvant, et en fonction de la charge, de la forme et de la taille de la molécule chargée. En outre, la puce peut contenir des détecteurs, tels que des détecteurs de polarisation de la lumière, des détecteurs d'émission de fluorescence, des biodétecteurs, des détecteurs électrochimiques ou d'autres microcomposants pouvant inclure des sites de manipulation enzymatique ou chimique des molécules chargées qui sont déplacées ou séparées.
Latest bibliographic data on file with the International Bureau