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1. WO1996041864 - DIODE LASER HEATED MICRO-REACTION CHAMBER WITH SAMPLE DETECTION MEANS

Publication Number WO/1996/041864
Publication Date 27.12.1996
International Application No. PCT/IB1996/000631
International Filing Date 10.06.1996
Chapter 2 Demand Filed 17.12.1996
IPC
B01L 3/00 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B01L 7/00 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
7Heating or cooling apparatus; Heat insulating devices
G01N 21/03 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
01Arrangements or apparatus for facilitating the optical investigation
03Cuvette constructions
CPC
B01L 2300/1861
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
18Means for temperature control
1861using radiation
B01L 2300/1872
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
18Means for temperature control
1861using radiation
1872Infrared light
B01L 3/502715
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5027by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
502715characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
B01L 7/52
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
7Heating or cooling apparatus
52with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
G01N 2021/0346
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
01Arrangements or apparatus for facilitating the optical investigation
03Cuvette constructions
0346Capillary cells; Microcells
G01N 21/03
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
01Arrangements or apparatus for facilitating the optical investigation
03Cuvette constructions
Applicants
  • THE REGENTS OF THE UNIVERSITY OF CALIFORNIA [US]/[US]
Inventors
  • NORTHRUP, Milton, A.
  • MARIELLA, Raymond, P., Jr.
  • KOO, Jackson, C.
  • DAVIDSON, J., Courtney
Agents
  • SARTORIO, Henry, P.
Priority Data
08/489,81913.06.1995US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) DIODE LASER HEATED MICRO-REACTION CHAMBER WITH SAMPLE DETECTION MEANS
(FR) CHAMBRE DE MICROREACTION CHAUFFEE PAR LASER A DIODE DOTEE D'UN MOYEN DE DETECTION D'ECHANTILLONS
Abstract
(EN)
A heat source (17, 18) and detector (19) arrangement for a miniaturized, microfabricated instrument (10) in which a polymerase chain reaction (PCR) is carried out. PCR is a bioanalytical method that uses enzymes and other reagents to amplify DNA or RNA from undetectable amounts to very detectable amounts, through a thermal cycling technique. Small IR or UV sources (17, 18) are used as heat sources for the PCR thermal cycle of the PCR technique, and sample conformation within the reaction chamber is established by detectors (19). Detection can be established when emitted light is absorbed by the DNA molecule, for example, or when a UV source induces fluorescence of the PCR products in the visible spectrum.
(FR)
La présente invention concerne un dispositif comprenant une source de chaleur (17, 18) et un détecteur (19), pour un instrument (10) microminiaturisé dans lequel a lieu une amplification enzymatique (PCR). La PCR est une méthode bioanalytique qui utilise des enzymes et d'autres réactifs pour faire amplifier l'ADN ou l'ARN de quantités indétectables à des quantités tout à fait détectables, et ce grâce à une technique de cycles thermiques. De petites sources d'infrarouge ou d'ultraviolet (17, 18) sont utilisées comme sources de chaleur pour le cycle thermique PCR de ladite technique, et la conformation des échantillons à l'intérieur de la chambre de réaction est établie par des détecteurs (19). La détection peut avoir lieu quand de la lumière émise est absorbée par la molécule d'ADN, par exemple, ou quand une source d'ultraviolet engendre la fluorescence des produits de la PCR dans le spectre visible.
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