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1. WO1996041186 - A METHOD FOR EARLY DETECTION OF HIV INFECTION IN BABIES

Note: Text based on automatic Optical Character Recognition processes. Please use the PDF version for legal matters

[ EN ]

Claims

1. A method for the early detection of HTV infection in a baby which comprises the steps of:
(a) contacting an immunoabsorbent coated with a protein having a high specific affinity for human IgA with the serum of a baby, and,
(b) detecting HIV-specific antibodies on said immunoabsorbent with an HTV-1 2 polypeptide operably linked with a signal generating system.

2. The method, according to claim 1, wherein said protein having high affinity for human IgA is protein B.

3. The method, according to claim 1, wherein the serum is from a baby of about 3 to about 4 months of age.

4. The method, according to claim 1, wherein the serum is from a baby less than 8 months of age.

5. The method, according to claim 1, wherein an amplification system is used to increase the sensitivity of the method.

6. The method, according to claim 5, wherein said amplification system is an enzyme amplification technique.

7. The method, according to claim 6, wherein said enzyme amplification technique comprises NADP as a substrate for said signal generating system, and the enzymes alcohol dehydrogenase and diaphorase.

8. A kit for the early detection of HTV infection in an infant comprising in one or more containers:
(a) a protein having a high specific binding affinity for human IgA and
(b) an HTV-12 polypeptide operably linked with a signal generating system.

9. The kit, according to claim 8, wherein said protein having high specific binding affinity is protein B.

10. The kit, according to claim 9, wherein said protein B is recombinantly produced.

11. The kit, according to claim 8, further comprising an amplification system that increases the sensitivity of the detection method.

12. The kit, according to claim 11, wherein said amplification system comprises an enzyme amplification technique.

13. The kit, according to claim 12, wherein said enzyme amplification technique comprises NADP as a substrate for an alkaline phosphatase signal generating system, and the enzymes alcohol dehydrogenase and diaphorase.