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1. WO1996041175 - ELECTROCHEMILUMINESCENT ENZYME IMMUNOASSAY

Publication Number WO/1996/041175
Publication Date 19.12.1996
International Application No. PCT/US1996/010119
International Filing Date 06.06.1996
Chapter 2 Demand Filed 09.12.1996
IPC
C07F 15/00 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
FACYCLIC, CARBOCYCLIC, OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
15Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic System
C07K 16/44 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins, e.g. monoclonal or polyclonal antibodies
44against material not provided for elsewhere
G01N 33/533 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
531Production of immunochemical test materials
532Production of labelled immunochemicals
533with fluorescent label
G01N 33/535 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
531Production of immunochemical test materials
532Production of labelled immunochemicals
535with enzyme label
CPC
C07F 15/0053
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
FACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
15Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic System
0006compounds of the platinum group
0046Ruthenium compounds
0053without a metal-carbon linkage
C07K 16/44
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
44against material not provided for elsewhere ; , e.g. haptens, metals, DNA, RNA, amino acids
G01N 33/533
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
531Production of immunochemical test materials
532Production of labelled immunochemicals
533with fluorescent label
G01N 33/535
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
531Production of immunochemical test materials
532Production of labelled immunochemicals
535with enzyme label ; or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
Applicants
  • IGEN, INC. [US]/[US]
Inventors
  • MARTIN, Mark, T.
  • SAUL, Richard
  • LIANG, Pam
Agents
  • RYAN, John, W.
Priority Data
08/484,76607.06.1995US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) ELECTROCHEMILUMINESCENT ENZYME IMMUNOASSAY
(FR) DOSAGE IMMUNOLOGIQUE D'ENZYMES PAR ELECTROCHIMIOLUMINESCENCE
Abstract
(EN)
Electrochemiluminescent-labels and enzyme substrates, which preferably are conjugated, are used in immunoassays and electrochemiluminescence is generated catalytically. In conventional electrochemiluminescence immunoassays, an anti-analyte antibody molecule can give rise to typically 6-8 electrochemiluminescence-active ruthenium atoms, while in the present invention, each enzyme-labeled anti-analyte molecule can give rise to thousands of electrochemiluminescence-active ruthenium atoms per second. An exemplary immunoassay is based on a catalytic process employing lactamase-conjugated anti-analytes which enzymatically hydrolyze electrochemiluminescent-labeled substrates, making them strongly electrochemiluminescent. The electrochemiluminescence signal generated by each anti-analyte molecule (i.e., each analyte molecule) is much greater than with the conventional method. Accordingly, greater sensitivity can be gained in the measurement of low concentrations of a given immunoassay analyte.
(FR)
Des marqueurs électrochimioluminescents et des substrats d'enzymes, de préférence conjugués, sont utilisés dans des dosages immunologiques et l'électrochimioluminescence est produite par voie catalytique. Dans les dosages immunologiques classiques par électrochimioluminescence, une molécule d'anticorps anti-analyte peut provoquer l'apparition, dans un cas typique, de 6 à 8 atomes de ruthénium électrochimioluminescents, tandis que, dans la présente invention, chaque molécule d'anticorps anti-analyte marquée par une enzyme peut provoquer l'apparition de milliers d'atomes de ruthénium électrochimioluminescents par seconde. Un dosage immunologique caractéristique a pour base un processus catalytique qui emploie des molécules d'anticorps anti-analyte conjugués à la lactamase, qui hydrolysent par voie enzymatique les substrats marqués par électrochimioluminescence, les rendant fortement électrochimioluminescents. Le signal d'électrochimioluminescence produit par chaque molécule d'anticorps anti-analyte (c'est-à-dire chaque molécule d'analyte) est beaucoup plus fort que celui produit avec le procédé classique. En conséquence, on peut obtenir une plus grande sensibilité de mesure de faibles concentrations d'un analyte donné dans un dosage immunologique.
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