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1. WO1996041015 - ENHANCEMENT OF ALKALINE PHOSPHATASE WITH SDS IN CHEMILUMINESCENT SUBSTRATES AND ENZYME INHIBITION ASSAYS

Publication Number WO/1996/041015
Publication Date 19.12.1996
International Application No. PCT/US1996/009767
International Filing Date 07.06.1996
Chapter 2 Demand Filed 15.11.1996
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
G01N 33/58 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
CPC
C12Q 1/6816
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
C12Q 1/6834
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
G01N 2333/916
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
90Enzymes; Proenzymes
914Hydrolases (3)
916acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
G01N 33/581
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
581with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
G01N 33/582
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
582with fluorescent label
Applicants
  • CHIRON CORPORATION [US]/[US]
Inventors
  • SHERIDAN, Patrick, J.
  • GAGNE, Julio, C.
  • ANDERSON, Mary, L.
  • LUDTKE, Douglas, N.
Agents
  • GOLDMAN, Kenneth, M.
Priority Data
08/472,75607.06.1995US
08/610,95505.03.1996US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) ENHANCEMENT OF ALKALINE PHOSPHATASE WITH SDS IN CHEMILUMINESCENT SUBSTRATES AND ENZYME INHIBITION ASSAYS
(FR) RENFORCEMENT DE PHOSPHATASES ALCALINES A L'AIDE DE SDS DANS DES SUBSTRATS CHIMIOLUMINESCENTS ET DES DOSAGES D'INHIBITION D'ENZYMES
Abstract
(EN)
A method of preparing a homogeneous alkaline phosphatase-oligonucleotide probe conjugate having high specific enzyme activity for use in nucleic acid hybridization assays is disclosed. Methods and compositions for enhancing the chemiluminescence from a stable 1,2-dioxetane triggered to produce a chemiluminescence are also disclosed. Indirect, competitive nucleic acid hybridization assay formats are also described that employ these methods and compositions.
(FR)
L'invention porte sur un procédé de préparation d'un conjugué sonde homogène de phosphatase alcaline/oligonucléotide, présentant une activité enzymatique spécifique, et qui s'utilise dans des dosages d'hybridation d'acides nucléiques; sur un procédé et des compositions renforçant la chimioluminescence d'une 1, 2 dioxétane stable pouvant être déclenchée pour produire la chimioluminescence; et sur des types de dosages d'hybridation d'acides nucléiques indirects et concurrents recourant à ces procédés et compositions.
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