Processing

Please wait...

Settings

Settings

Goto Application

1. WO1996041013 - METHOD FOR SCREENING FOR RECEPTOR AGONISTS AND ANTAGONISTS

Publication Number WO/1996/041013
Publication Date 19.12.1996
International Application No. PCT/US1996/009638
International Filing Date 06.06.1996
Chapter 2 Demand Filed 03.01.1997
IPC
C07K 14/51 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
475Growth factors; Growth regulators
51Bone morphogenic factor; Osteogenin; Osteogenic factor; Bone-inducing factor
C07K 14/71 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
71for growth factors; for growth regulators
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
G01N 33/50 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G01N 33/567 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
566using specific carrier or receptor proteins as ligand binding reagent
567utilising isolate of tissue or organ as binding agent
CPC
C07K 14/51
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
475Growth factors; Growth regulators
51Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
C07K 14/71
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
71for growth factors; for growth regulators
C12Q 1/6897
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6897involving reporter genes operably linked to promoters
G01N 33/5005
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
5005involving human or animal cells
G01N 33/567
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
566using specific carrier or receptor proteins as ligand binding reagents ; where possible specific carrier or receptor proteins are classified with their target compounds
567utilising isolate of tissue or organ as binding agent
Applicants
  • LIGAND PHARMACEUTICALS INCORPORATED [US]/[US]
Inventors
  • McDONNELL, Donald, P.
  • TZUKERMAN, Maty
  • DELORME, Evelyn
  • MINER, Jeffrey, N.
  • GLEESON, Martin, A., G.
  • WEN, Xiaohong, Dawn
  • PIKE, J., Wesley
Agents
  • CHEN, Anthony, C.
  • MOON, Donald, Keith
Priority Data
08/482,43607.06.1995US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD FOR SCREENING FOR RECEPTOR AGONISTS AND ANTAGONISTS
(FR) PROCEDES DE DEPISTAGE D'AGONISTES ET D'ANTAGONISTES DE RECEPTEURS
Abstract
(EN)
This invention relates to a method for identifying a receptor agonist or antagonist by providing a nucleic acid encoding a receptor having a first TAF region able to activate transcription from a promotor, and a second TAF region mutated to have the functional context of the second TAF region, but not able on its own to activate transcription of the promoter. The nucleic acid is provided within a cell unable to exhibit transcription from the promoter in the presence of the second TAF region alone, but able to exhibit transcription from the promoter in the presence of the first TAF region. The cell further includes a reporter construct containing the promoter operatively linked to a reporter gene. The reporter gene is transcribed when the promoter is activated in the presence of the first TAF region. The method further includes contacting the cell with a potential agonist or antagonist, under conditions in which contact of the cell with a known agonist or antagonist of the receptor increases or decreases transcription from the promoter and the level of the product of the reporter construct. Finally, the method involves measuring the level of increase of the product of the reporter construct as an indication of the agonist activity of the potential agonist. The method is applicable to intracellular receptors including ER, AR, PR and GR.
(FR)
L'invention concerne un procédé pour identifier un agoniste ou un antagoniste d'un récepteur en utilisant un acide nucléique codant pour un récepteur ayant une première région TAF capable d'activer la transcription depuis un promoteur et une seconde région TAF mutée pour avoir le contexte fonctionnel de la seconde région TAF, mais incapable toute seule d'activer la transcription du promoteur. L'acide nucléique se trouve dans une cellule incapable d'assurer la transcription depuis le promoteur en présence de la seule seconde région TAF, mais il peut assurer la transcription depuis le promoteur en présence de la première région TAF. La cellule contient, en outre, un reporter de recombinaison contenant le promoteur lié d'une manière opérationnelle au gène reporter. Le gène reporter est transcrit quand le promoteur est activé en présence de la première région TAF. Le procédé consiste, en outre, à mettre la cellule en contact avec un agoniste ou un antagoniste potentiel, dans des conditions où le contact de la cellule avec un agoniste ou un antagoniste connu du récepteur augmente ou diminue la transcription depuis le promoteur et le niveau du produit du reporter de recombinaison. Finalement, le procédé consiste à mesurer le niveau d'augmentation du produit du reporter de recombinaison pour avoir une indication de l'activité de l'agoniste potentiel. Le procédé est applicable à des récepteurs intracellulaires tels que ER, AR, PR et GR.
Also published as
Latest bibliographic data on file with the International Bureau