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1. WO1996040990 - METHODS AND KITS FOR DETERMINING PRE-AMPLIFICATION LEVELS OF A NUCLEIC ACID TARGET SEQUENCE FROM POST-AMPLIFICATION LEVELS OF PRODUCT

Publication Number WO/1996/040990
Publication Date 19.12.1996
International Application No. PCT/US1996/006300
International Filing Date 02.05.1996
Chapter 2 Demand Filed 31.12.1996
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/6851
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
6851Quantitative amplification
C12Q 1/6865
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
6865Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
Applicants
  • GEN-PROBE INCORPORATED [US]/[US]
Inventors
  • RYDER, Thomas, Brendan
  • SHANNON, Karen, W.
  • KACIAN, Daniel, Louis
  • HARVEY, Richard, C.
  • McDONOUGH, Sherrol, H.
  • GONZALES, Frank, R.
  • CASTILLO, Maria, R.
  • BILLYARD, Elizabeth, R.
  • SHEN, Nancy, Lau, Liu
Agents
  • SCHNEIDER, Carol, A.
  • VIERING, JENTSCHURA & PARTNER
Priority Data
08/482,42807.06.1995US
08/486,70507.06.1995US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS AND KITS FOR DETERMINING PRE-AMPLIFICATION LEVELS OF A NUCLEIC ACID TARGET SEQUENCE FROM POST-AMPLIFICATION LEVELS OF PRODUCT
(FR) PROCEDES ET TROUSSES SERVANT A DETERMINER LES NIVEAUX DE PREAMPLIFICATION D'UNE SEQUENCE CIBLE D'ACIDES NUCLEIQUES A PARTIR DES NIVEAUX DE POST-AMPLIFICATION DU PRODUIT
Abstract
(EN)
Methods and kits for relating initial amounts of target nucleic acids present in a sample to target-specific amplification products. It has been discovered that the transcription-mediated amplification system is capable of producing a quantitative relationship between target input and target-specific output. Further, the present invention relates to methods for carefully controlling this relationship resulting in an unexpectedly high degree of reproducibility. Also described are useful methods for extending the dynamic range of transcription-based amplification systems.
(FR)
Procédés et trousses permettant de mettre en rapport les quantités initiales d'acides nucléiques cibles présentes dans un échantillon avec des produits d'amplification à cible spécifique. On a découvert que le système d'amplification à médiation de transcription est à même de produire un rapport quantitatif entre une entrée cible et une sortie spécifique à une cible. En outre, l'invention se rapporte à des procédés permettant de modules soigneusement ce rapport, avec pour résultat inattendu un taux élevé de reproductibilité. L'invention concerne également des procédés qui permettent d'étendre la plage dynamique des systèmes d'amplification à base de transcription.
Also published as
KR1019970709080
Latest bibliographic data on file with the International Bureau