Processing

Please wait...

Settings

Settings

Goto Application

1. WO1996039525 - CLONING AND EXPRESSION OF THERMOSTABLE MUTS GENES AND PROTEINS AND USES THEREFOR

Publication Number WO/1996/039525
Publication Date 12.12.1996
International Application No. PCT/US1996/008677
International Filing Date 04.06.1996
Chapter 2 Demand Filed 30.12.1996
IPC
C07K 14/195 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C07K 14/195
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
C12Q 1/6844
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
C12Q 1/6848
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
6848characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
C12Q 1/6858
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
6858Allele-specific amplification
C12Q 1/686
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
686Polymerase chain reaction [PCR]
Y10T 436/143333
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
436Chemistry: analytical and immunological testing
14Heterocyclic carbon compound [i.e. , O, S, N, Se, Te, as only ring hetero atom]
142222Hetero-O [e.g., ascorbic acid, etc.]
143333Saccharide [e.g., DNA, etc.]
Applicants
  • THE MOUNT SINAI MEDICAL CENTER OF THE CITY UNIVERSITY OF NEW YORK [US]/[US]
Inventors
  • WETMUR, James, G.
Agents
  • GRANAHAN, Patricia
Priority Data
08/468,55806.06.1995US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CLONING AND EXPRESSION OF THERMOSTABLE MUTS GENES AND PROTEINS AND USES THEREFOR
(FR) CLONAGE ET EXPRESSION DE MUTANTS THERMOSTABLES DE GENES ET DE PROTEINES, ET LEURS UTILISATIONS
Abstract
(EN)
Isolated nucleic acids which encode a thermostable protein which binds specifically to bulge loops in a heteroduplex nucleic acid and recombinant vectors comprising nucleic acid which encodes a thermostable protein which binds specifically to bulge loops in a heteroduplex nucleic acid are disclosed. Also disclosed are isolated thermostable proteins which bind specifically to bulge loops in a heteroduplex nucleic acid and host cells comprising a recombinant gene which can express a thermostable protein which binds specifically to bulge loops in a heteroduplex nucleic acid. Further disclosed are a method of reducing DNA misincorporation in an amplification reaction, methods for detecting a nucleic acid which includes a specific sequence, a method for amplifying a nucleic acid comprising a specific sequence, and a method for selecting against a nucleic acid comprising a specific sequence.
(FR)
L'invention porte sur des acides nucléiques isolés codant pour une protéine thermostable se fixant spécifiquement aux protubérances en boucle d'un acide nucléique d'hétéroduplex et sur des vecteurs de recombinaison comportant un acide nucléique se fixant spécifiquement aux protubérances en boucle d'un acide nucléique d'hétéroduplex. Elle porte également sur des protéines thermostables se fixant spécifiquement aux protubérances en boucle d'un acide nucléique d'hétéroduplex et sur des cellules hôtes comportan t un gène de recombinaison capable d'exprimer une protéine thermostable se fixant spécifiquement aux protubérances en boucle d'un acide nucléique d'hétéroduplex, sur un procédé de détection des défauts d'incorporation de l'ADN dans une réaction d'amplification, sur des procédés de détection d'un acide nucléique comportant une séquence spécifique, sur un procédé d'amplification d'un acide nucléique comprenant une séquence spécifique, et sur un procédé de resélection d'un acide nucléique comportant une séquence spécifique.
Latest bibliographic data on file with the International Bureau