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1. WO1996039489 - ISOLATION OF MAMMALIAN HEMATOPOIETIC STEM CELLS

Publication Number WO/1996/039489
Publication Date 12.12.1996
International Application No. PCT/US1996/009415
International Filing Date 05.06.1996
Chapter 2 Demand Filed 03.01.1997
IPC
A61K 35/28 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
28Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
C12N 5/0789 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
078Cells from blood or from the immune system
0789Stem cells; Multipotent progenitor cells
A61K 38/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
CPC
A61K 35/28
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
28Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
A61K 38/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
C12N 5/0647
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0634Cells from the blood or the immune system
0647Haematopoietic stem cells; Uncommitted or multipotent progenitors
Applicants
  • WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH [US]/[US] (AllExceptUS)
  • MASSACHUSETTS INSTITUTE OF TECHNOLOGY [US]/[US] (AllExceptUS)
  • GOODELL, Margaret, A. [US]/[US] (UsOnly)
  • CONNER, A., Stewart [GB]/[US] (UsOnly)
  • MULLIGAN, Richard, C. [US]/[US] (UsOnly)
Inventors
  • GOODELL, Margaret, A.
  • CONNER, A., Stewart
  • MULLIGAN, Richard, C.
Agents
  • GRANAHAN, Patricia
Priority Data
08/471,75806.06.1995US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) ISOLATION OF MAMMALIAN HEMATOPOIETIC STEM CELLS
(FR) ISOLATION DE CELLULES SOUCHES HEMATOPOÏETIQUES DE MAMMALIENNES
Abstract
(EN)
The present invention relates to a method of purifying mammalian hematopoietic stem cells from a bone marrow cell population. The present invention also relates to a method of separating cycling HSCs from quiescent HSCs in a purified mammalian HSC sample. Further, the invention relates to a method of identifying an agent which is a fluorescent, vital dye which is lipophilic for use in purifying mammalian HSCs. The present invention also relates to a method of transplanting bone marrow in a mammalian host, such as a human, comprising introducing into the host the purified HSCs described herein. The invention further relates to a method of $i(in vivo) administration of a protein comprising transfecting a purified HSC with a construct comprising DNA which encodes a protein of interest and then introducing the stem cell into the host where the protein of interest is expressed. The present invention also relates to hematopoietic stem cells purified using the methods described herein.
(FR)
Un procédé permet de purifier des cellules souches hématopoïétiques mammaliennes provenant d'une population de cellules de moëlle osseuse. Un autre procédé permet de séparer des cellules souches hématopoïétiques actives de celles qui sont quiescentes dans un échantillon de ces cellules purifiées mammaliennes. On décrit aussi un procédé d'identification d'un agent qui est un colorant vital fluorescent et lipophile qu'on utilise pour purifier ces cellules mammaliennes, ainsi qu'un procédé de greffe de moëlle osseuse chez un mammifère, tel qu'un être humain, procédé qui consiste à introduire chez l'hôte les cellules souches hématopoïétiques purifiées décrites. On décrit en outre un procédé d'administration $i(in vivo) d'une protéine, qui consiste à transfecter une cellule souche hématopoïétique purifiée avec un produit d'assemblage comprenant de l'ADN qui code une protéine souhaitée, puis à introduire cette cellule souche chez l'hôte où cette protéine s'exprime. On décrit aussi des cellules souches hématopoïétiques purifiées à l'aide des procédés décrits.
Also published as
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