Processing

Please wait...

Settings

Settings

Goto Application

1. WO1996038725 - ATTACHMENT ENHANCED 293 CELLS

Note: Text based on automatic Optical Character Recognition processes. Please use the PDF version for legal matters

[ EN ]

WHAT IS CLAIMED IS:

1. Human embryonic kidney 293 cells transfected with a mammalian scavenger receptor gene, said cells demonstrating an enhanced ability to attach to a solid support.
2. The cells according to claim 1 wherein said receptor gene is a human macrophage scavenger receptor gene Type I.
3. The cells according to claim 1 wherein the receptor gene is characterized by the sequence of GenBank accession number D90187.
4. The cells according to claim 1 wherein said receptor gene is a human macrophage scavenger receptor gene Type II.
5. The cells according to claim 1 wherein the receptor gene is characterized by the sequence of GenBank accession number D90188.
6. The cells according to claim 1 wherein said receptor gene is a macrophage scavenger receptor gene of a non-human species.
7. A solid support to which is attached human embryonic kidney 293 cells transfected with a mammalian scavenger receptor gene.
8. The support according to claim 7- wherein said receptor gene is a human macrophage scavenger receptor gene Type I.
9. The support according to claim 7 wherein the receptor gene is characterized by the sequence of GenBank accession number D90187.
10. The support according to claim 7 wherein said receptor gene is a human macrophage scavenger receptor gene Type II.
11. The support according to claim 7 wherein the receptor gene is characterized by the sequence of GenBank accession number D90188.
12. The support according to claim 7 wherein said receptor gene is a macrophage scavenger receptor gene of a non-human species.
13. A method of enhancing the ability of human embryonic kidney cells to attach to a solid support comprising the steps of:
(a) providing cells from a 293 cell line; and (b) transfecting the cells with a mammalian scavenger receptor gene;
wherein the transfected cells are characterized by an enhanced ability to attach to said solid support.
14. The method according to claim 13 further comprising transfecting said cells with a second selected gene.
15. The method according to claim 14 wherein the selected gene is a selection marker.
16. The method according to claim 15 wherein the gene is a selectable resistance marker.
17. A method of screening a compounds for biological activity comprising the steps of:
(a) providing on a solid support human embryonic kidney 293 cells co-transfected with a mammalian scavenger receptor gene and a second selected gene which encodes a protein having a biological activity;
(b) measuring expression of the protein encoded by said second selected gene;
(c) incubating said co-transfected 293 cells in the presence of a compound of unknown biological activity;
(d) screening the cells of (c) for the ability of the compound to alter said biological activity.
18. The method according to claim 17 wherein the receptor gene is a human macrophage scavenger receptor gene Type I or Type II.
19. An improved method for screening a compound for biological activity comprising measuring in a transfected cell the expression of a protein encoded by a selected gene; incubating said transfected cell in the presence of a compound of unknown biological activity; and screening the cell for the ability of said compound to alter said biological activity, the improvement comprising employing as said transfected cell, human embryonic kidney 293 cells co-transfected with a mammalian scavenger receptor gene and said selected gene, said cells attached to a solid support.

20. An improved method for performing a biological assay on a cell attached to a solid support, wherein said assay involves at least one washing step, said improvement comprising employing as said attached cell, human embryonic kidney 293 cells co-transfected with a mammalian scavenger receptor gene.
21. An improved method for measuring the production of a protein in a cell attached to a solid support, said improvement comprising employing as said attached cell, human embryonic kidney 293 cells co-transfected with a mammalian scavenger receptor gene.