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1. WO1996038584 - ASSAY FOR MICROORGANISMS

Publication Number WO/1996/038584
Publication Date 05.12.1996
International Application No. PCT/GB1996/001296
International Filing Date 31.05.1996
Chapter 2 Demand Filed 02.12.1996
IPC
C12Q 1/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C12Q 1/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
10Enterobacteria
CPC
C12Q 1/045
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
045Culture media therefor
C12Q 1/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
10Enterobacteria
C12Q 2334/50
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2334O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
50Indoles
C12Q 2334/52
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2334O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
50Indoles
525-Bromo-4-chloro-3-indolyl, i.e. BCI
Applicants
  • CELSIS INTERNATIONAL PLC [GB]/[GB] (AllExceptUS)
  • GRANT, Peter, Leonard [GB]/[GB] (UsOnly)
  • FORSTER, Simon, James [GB]/[GB] (UsOnly)
  • THOMAS, Brian [GB]/[GB] (UsOnly)
Inventors
  • GRANT, Peter, Leonard
  • FORSTER, Simon, James
  • THOMAS, Brian
Agents
  • GILL JENNINGS & EVERY
Priority Data
9510928.631.05.1995GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) ASSAY FOR MICROORGANISMS
(FR) DETECTION DE MICRO-ORGANISMES
Abstract
(EN)
In an assay for coliform or other microorganisms in a sample, which comprises contacting a mixture of the sample and a liquid growth medium with a material that undergoes a detectable change in the presence of the microorganisms to be assayed, the sample is contained in a well whose base is defined by a filter material, and the mixture is formed by placing the well on the growth medium. A preferred embodiment additionally comprises removing the sample and conducting a further assay adapted to distinguish between the microorganisms to be assayed and any other that may have given a false positive.
(FR)
Dans une bioanalyse relative aux coliformes et autres micro-organismes d'un échantillon, consistant à mettre en présence un mélange de l'échantillon et un produit subissant une modification décelable en présence du micro-organisme à analyser, l'échantillon est placé dans un puits dont la base est faite d'un matériau filtrant, et le mélange s'obtient en plaçant le puits sur le milieu de culture. L'une des variantes préférée consiste à retirer l'échantillon et à effectuer une autre analyse conçue pour distinguer entre les micro-organismes à tester et tout autre ayant pu donner un faux positif.
Also published as
Latest bibliographic data on file with the International Bureau