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1. WO1996038561 - IMPROVED METHODS OF PRODUCING FEED BY REDUCING ENDOGENOUS PROTEIN LEVELS IN SOYBEAN

Publication Number WO/1996/038561
Publication Date 05.12.1996
International Application No. PCT/US1996/008207
International Filing Date 31.05.1996
Chapter 2 Demand Filed 27.12.1996
IPC
A01H 1/06 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1Processes for modifying genotypes
06Processes for producing mutations, e.g. treatment with chemicals or with radiation
A01H 5/10 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
10Seeds
C07K 14/415 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
415from plants
C12N 15/82 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
CPC
A01H 1/06
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1Processes for modifying genotypes
06Processes for producing mutations, e.g. treatment with chemicals or with radiation
A01H 5/10
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
10Seeds
C07K 14/415
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
415from plants
C12N 15/8242
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8242with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
C12N 15/8253
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8242with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
8243involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
8253Methionine or cysteine
C12N 15/8254
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8242with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
8243involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
8254Tryptophan or lysine
Applicants
  • PIONEER HI-BRED INTERNATIONAL, INC. [US]/[US]
Inventors
  • BEACH, Larry, R.
Agents
  • SIMON, Soma, G.
  • JERVIS, Herbert
Priority Data
08/459,98902.06.1995US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) IMPROVED METHODS OF PRODUCING FEED BY REDUCING ENDOGENOUS PROTEIN LEVELS IN SOYBEAN
(FR) PROCEDES PERFECTIONNES DE PRODUCTION D'ALIMENTS PAR REDUCTION DE LA TENEUR EN PROTEINES ENDOGENES DU SOJA
Abstract
(EN)
The present invention provides a plant seed that is genetically modified, relative to a wild type of the species of the seed, to preferentially express a preselected protein whereby the content of other endogenous proteins is diminished in the seed. The plant seed is further modified to have reduced levels of trypsin and/or chymotrypsin inhibitor activity. The preselected protein is preferably a methionine-rich 2S seed storage protein, Brazil nut protein (BNP). The invention is based on the discovery that the combination of the traits of BNP and reduced levels of KTI, BBI and/or GRSTI into the same cultivar leads to an unexpectedly high reduction or elimination of inhibitor activity in the seed. Thus, the present method eliminates the need for costly processing which can damage protein products.
(FR)
La présente invention concerne une semence végétale génétiquement modifiée, par rapport à un type sauvage de l'espèce de cette semence, pour exprimer de préférence une protéine présélectionnée permettant de réduire la teneur de la semence en d'autres protéines endogènes. Cette semence végétale est également modifiée pour présenter des niveaux réduits d'activité d'inhibiteur de la trypsine et/ou de la chymotrypsine. La protéine présélectionnée est de préférence une protéine de réserve séminale 2S riche en méthionine, la protéine de la noix du Brésil (PNB). L'invention est fondée sur la découverte que l'association des caractéristiques de la PNB et de niveaux réduits de KTI, BBI et/ou GRSTI dans le même cultivar entraîne une réduction étonnamment élevée ou une élimination de l'activité d'inhibiteur dans la semence. Ainsi, le procédé décrit élimine la nécessité d'un traitement coûteux susceptible d'endommager les produits protéiques.
Also published as
Latest bibliographic data on file with the International Bureau