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1. WO1996032646 - PROCESS FOR DETECTING HIV-TAT

Publication Number WO/1996/032646
Publication Date 17.10.1996
International Application No. PCT/DE1996/000641
International Filing Date 12.04.1996
Chapter 2 Demand Filed 13.11.1996
IPC
G01N 33/569 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
CPC
G01N 33/56988
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
56983Viruses
56988AIDS or HTLV
Applicants
  • DEUTSCHES KREBSFORSCHUNGSZENTRUM STIFTUNG DES ÖFFENTLICHEN RECHTS [DE]/[DE] (AllExceptUS)
  • KRAMMER, Peter [DE]/[DE] (UsOnly)
  • WESTENDORP, Michael [DE]/[DE] (UsOnly)
  • FRANK, Rainer [DE]/[DE] (UsOnly)
  • BERNDT, Christina [DE]/[DE] (UsOnly)
Inventors
  • KRAMMER, Peter
  • WESTENDORP, Michael
  • FRANK, Rainer
  • BERNDT, Christina
Agents
  • HUBER, Bernard
Priority Data
195 14 089.313.04.1995DE
Publication Language German (DE)
Filing Language German (DE)
Designated States
Title
(DE) NACHWEISVERFAHREN FÜR HIV-TAT
(EN) PROCESS FOR DETECTING HIV-TAT
(FR) PROCEDE DE DEPISTAGE DU VIH-TAT
Abstract
(DE)
Die Erfindung betrifft ein Verfahren zum Nachweis von HIV-TAT in einer Körperprobe, umfassend die folgenden Verfahrensschritte: (a) Entnahme einer Körperprobe und Inkubation dieser mit einem Detergens und hohem Salz, (b) Größenfraktionierung der Körperprobe von (a), und (c) Inkubation der Körperprobe von (b) mit einem Anti-HIV-TAT-Antikörper. Ferner betrifft die Erfindung einen zur Durchführung des Verfahrens geeigneten Kit.
(EN)
The invention relates to a process for detecting HIV-TAT in a body sample comprising the steps: (a) taking a body sample and incubating it with a detergent and a high salt, (b) size-fractionating the body sample from (a), and (c) incubation of the body sample from (b) with an anti-HIV-TAT antibody. The invention also relates to a kit suitable for implementing the process.
(FR)
L'invention concerne un procédé de dépistage du VIH-TAT dans un échantillon prélevé sur le corps, ledit procédé comprenant les étapes suivantes: (a) prélèvement d'un échantillon sur le corps et incubation de l'échantillon avec un détergent et un sel concentré; (b) fractionnement de la taille de l'échantillon de l'étape (a); (c) incubation de l'échantillon de l'étape (b) avec un anticorps anti-VIH-TAT. L'invention concerne en outre une trousse permettant de mettre en ÷uvre ledit procédé.
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