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1. WO1996019501 - MODULATORS OF BONE CELL FUNCTION AND USES THEREOF

Publication Number WO/1996/019501
Publication Date 27.06.1996
International Application No. PCT/US1995/016826
International Filing Date 19.12.1995
IPC
A61K 38/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
C07K 14/51 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
475Growth factors; Growth regulators
51Bone morphogenic factor; Osteogenin; Osteogenic factor; Bone-inducing factor
C07K 16/22 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins, e.g. monoclonal or polyclonal antibodies
18against material from animals or humans
22against growth factors
C12N 9/66 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
48acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
66Elastase
CPC
A61K 38/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
C07K 14/51
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
475Growth factors; Growth regulators
51Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
C07K 16/22
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
18against material from animals or humans
22against growth factors ; ; against growth regulators
C12N 9/6448
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
48acting on peptide bonds (3.4)
50Proteinases ; , e.g. Endopeptidases (3.4.21-3.4.25)
64derived from animal tissue
6421from mammals
6424Serine endopeptidases (3.4.21)
6448Elastases, e.g. pancreatic elastase (3.4.21.36); leukocyte elastase (3.4.31.37)
Applicants
  • OSTEOSA INC. [US]/[US] (AllExceptUS)
  • YONEDA, Toshiyuki [JP]/[US] (UsOnly)
  • IZBICKA, Elzbieta [US]/[US] (UsOnly)
  • MUNDY, Gregory, R. [US]/[US] (UsOnly)
Inventors
  • YONEDA, Toshiyuki
  • IZBICKA, Elzbieta
  • MUNDY, Gregory, R.
Agents
  • PARKER, David, L.
Priority Data
08/363,09220.12.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MODULATORS OF BONE CELL FUNCTION AND USES THEREOF
(FR) MODULATEURS DES FONCTIONS DES CELLULES OSSEUSES ET LEURS UTILISATIONS
Abstract
(EN)
A pancreas-derived factor has been identified which lowers serum calcium and inhibits bone resorption, and also stimulates proliferation of MG-63 osteoblast-like cells. The factor has been purified to homogeneity from porcine pancreas and obtained definitive amino acid sequence has been obtained (SEQ ID NO:1). This factor bears homology to a human pancreatic elastase, elastase IIIB. Experiments with recombinant human pancreatic elastase IIIB as well as neutralizing antibodies to human pancreatic elastase IIIB demonstrate that the biological effects of native PX are mimicked by those of recombinant human elastase IIIB both $i(in vitro) and $i(in vivo). Numerous diagnostic and therapeutic applications are contemplated, including drug screening assays, test kits for diagnosing and monitoring conditions characterized by changes in bone cell population, and compositions and methods for treating undesirable bone resorption or accretion.
(FR)
On a identifié un facteur dérivé du pancréas qui diminue le calcium sérique, qui inhibe la résorption osseuse et qui également stimule la prolifération des cellules MG-63, qui sont des cellules du type ostéoblastes. Ce facteur a été purifié jusqu'à obtenir un produit homogène, en partant de pancréas de porcs, et on a déterminé la séquence définitive de ses acides aminés (SEQ ID NO:1). Ce facteur et l'élastase BIII (qui est une élastase pancréatique humaine) sont homologues. Des expériences avec l'élastase pancréatique humaine IIIB de recombinaison, ainsi qu'avec des anticorps anti-élastase pancréatique humaine IIIB démontrent que les effets biologiques du facteur PX natif sont également obtenus par l'élastase pancréatique humaine IIIB de recombinaison, aussi bien in vitro que in vivo. De nombreuses applications diagnostiques et thérapeutiques sont envisagées, en particulier l'évaluation préliminaire de médicaments et la réalisation de coffrets de réactifs pour diagnostiquer et surveiller les changements dans les populations de cellules osseuses. L'invention concerne également des compositions et des procédés pour traiter des résorptions ou des accrétions osseuses indésirables.
Also published as
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