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1. WO1996017952 - METHOD AND APPARATUS FOR THE PRECISE POSITIONING OF CELLS

Publication Number WO/1996/017952
Publication Date 13.06.1996
International Application No. PCT/US1995/016310
International Filing Date 08.12.1995
Chapter 2 Demand Filed 02.07.1996
IPC
C12M 1/26 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
26Inoculator or sampler
C12M 1/32 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
26Inoculator or sampler
32multiple field or continuous type
C12M 3/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
04with means providing thin layers
G01N 1/28 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
G01N 15/14 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation
CPC
C12M 21/08
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
21Bioreactors or fermenters specially adapted for specific uses
08for producing artificial tissue or for ex-vivo cultivation of tissue
G01N 1/2813
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
2813Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
G01N 2001/282
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
2813Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
282with mapping; Identification of areas; Spatial correlated pattern
G01N 2015/149
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
149Sorting the particles
Applicants
  • BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM [US]/[US]
Inventors
  • KLEBE, Robert, J.
Agents
  • PETERMAN, Brian, W.
Priority Data
08/352,27609.12.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD AND APPARATUS FOR THE PRECISE POSITIONING OF CELLS
(FR) PROCEDE ET APPAREIL PERMETTANT LE POSITIONNEMENT PRECIS DE CELLULES
Abstract
(EN)
Disclosed are a method and apparatus (100) for the precise positioning of cells into highly organized tissue structures, which are suitable for use in the treatment of burn patients. Specific placement of the cells onto substrata (130) is obtained by the computer-controlled direct placement of cell suspension matrix material, which includes cells suspended by an extracellular matrix material, including cell adhesion proteins, collagens, gelatin, and cyanoacrylates or other cellular glues. A fluorescence activated cell sorter (148) is used to provide a stream or droplet stream (150) of the biological material. Cells may also be positioned by computer-controller placement of cell adhesion materials such as fibronectin to a substratum (130) which is suitable for maintaining cell growth, followed by allowing cells to attach to those areas of the substratum (130) where the cell adhesion material has been placed. These apparati may even be further modified and improved by providing a controlled biological environment in the area of the substratum (130), thus providing an integral unit for the production of organized tissues.
(FR)
Un procédé et un appareil (100) permettent le positionnement précis de cellules dans des structures de tissus très organisés convenant pour traiter des victimes de brûlures. On obtient un positionnement spécifique de cellules sur des substrats (130) grâce au placement direct informatisé d'un matériau d'une matrice de suspension cellulaire, qui comprend des cellules en suspension dans un matériau de matrice extracellulaire, y compris des protéines d'adhérence cellulaire, des collagènes, des gélatines et des cyanoacrylates ou d'autres colles cellulaires. Un trieur de cellules à fluorescence (148) produit un flux ou un flux de gouttelettes (150) d'un matériau biologique. Des cellules peuvent aussi être positionnées par un positionnement informatisé de matériaux d'adhérence cellulaire, tels qu'une fibronectine, sur un substrat (130) convenant pour la croissance cellulaire, puis en permettant à ces cellules de se fixer sur des zones du substrat (130) où l'on a déposé le matériau d'adhérence cellulaire. Un tel appareil peut même être encore modifié et amélioré par l'utilisation d'un environnement biologique maîtrisé dans la zone du substrat (130), ce qui procure une installation intégrée destinée à produire des tissus organisés.
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