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1. WO1996006191 - PRIMERS AND PROBES FOR THE AMPLIFICATION AND DETECTION OF CMV NUCLEIC ACID

Publication Number WO/1996/006191
Publication Date 29.02.1996
International Application No. PCT/EP1995/003295
International Filing Date 18.08.1995
IPC
C12Q 1/70 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
CPC
C12Q 1/701
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
70involving virus or bacteriophage
701Specific hybridization probes
C12Q 1/705
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
70involving virus or bacteriophage
701Specific hybridization probes
705for herpetoviridae, e.g. herpes simplex, varicella zoster
Applicants
  • AKZO NOBEL N.V. [NL/NL]; Velperweg 76 NL-6824 BM Arnhem, NL (AllExceptUS)
  • SILLEKENS, Peter, Theodorus, Gerardus [NL/NL]; NL (UsOnly)
  • TIMMERMANS, Eveline, Catharina, Anna, Clasina [NL/NL]; NL (UsOnly)
Inventors
  • SILLEKENS, Peter, Theodorus, Gerardus; NL
  • TIMMERMANS, Eveline, Catharina, Anna, Clasina; NL
Agents
  • BEETZ, T.; Postbus 20 NL-5340 BH Oss, NL
Priority Data
94202360.718.08.1994EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PRIMERS AND PROBES FOR THE AMPLIFICATION AND DETECTION OF CMV NUCLEIC ACID
(FR) AMORCES ET SONDES DESTINEES A L'AMPLIFICATION ET A LA DETECTION DE L'ACIDE NUCLEIQUE DU CYTOMEGALOVIRUS
Abstract
(EN)
The present invention relates to a method for the diagnosis of symptomatic CMV disease, characterized in that the presence of mRNA encoding a late structural protein of the human Cytomegalovirus in a blood sample of an individual, suspected of carrying said disease, is detected, said method comprising the following steps: amplifying a target sequence within said mRNA using a primer pair capable of specifically reacting with said target sequence and suitable amplification reagents, reacting the sample, optionally containing amplified nucleic acid, with a labeled nucleic acid probe having a sequence complementary to part of the target sequence, detecting hybrids formed between the target sequence and the probe. The present invention further provides primers and probes for the amplification and detection of late pp67 HCMV mRNA.
(FR)
L'invention concerne un procédé de diagnostic de la maladie symptomatique à cytomégalovirus, caractérisé en ce qu'il permet de détecter la présence de l'ARNm codant une protéine structurelle tardive du cytomégalovirus humain dans un échantillon sanguin d'un individu, soupçonné d'être porteur de cette maladie, ledit procédé comprenant les étapes suivantes consistant: - à amplifier une séquence cible dans ledit ARNm à l'aide d'une paire d'amorces susceptible de réagir de manière spécifique avec la séquence cible ainsi qu'avec des réactifs d'amplification appropriés; - à faire réagir l'échantillon, contenant éventuellement l'acide nucléique amplifié, avec une sonde d'acide nucléique marquée possédant une séquence complémentaire afin de séparer la séquence cible; - et à détecter les hybrides formés entre la séquence cible et la sonde. L'invention décrit en outre des amorces et des sondes destinées à l'amplification et à la détection de l'ARNm du cytomégalovirus humain pp67 tardif.
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