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1. WO1996006189 - CAPILLARY ELECTROPHORETIC DETECTION OF NUCLEIC ACIDS

Publication Number WO/1996/006189
Publication Date 29.02.1996
International Application No. PCT/US1995/010587
International Filing Date 21.08.1995
Chapter 2 Demand Filed 22.03.1996
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
G01N 27/447 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
CPC
C12Q 1/68
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
C12Q 1/6813
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
G01N 27/44747
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
44704Details; Accessories
44747Composition of gel or of carrier mixture
Applicants
  • ISIS PHARMACEUTICALS, INC. [US/US]; 2280 Faraday Avenue Carlsbad, CA 92008, US (AllExceptUS)
  • CUMMINS, Lendell, L. [US/US]; US (UsOnly)
  • FREIER, Susan, M. [US/US]; US (UsOnly)
  • GRIFFEY, Richard [US/US]; US (UsOnly)
  • SRIVATSA, G., Susan [US/US]; US (UsOnly)
Inventors
  • CUMMINS, Lendell, L.; US
  • FREIER, Susan, M.; US
  • GRIFFEY, Richard; US
  • SRIVATSA, G., Susan; US
Agents
  • CALDWELL, John, W. ; Woodcock Washburn Kurtz Mackiewicz & Norris 46th floor One Liberty Place Philadelphia, PA 19103, US
Priority Data
08/295,50924.08.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CAPILLARY ELECTROPHORETIC DETECTION OF NUCLEIC ACIDS
(FR) DETECTION D'ACIDES NUCLEIQUES PAR ELECTROPHORESE CAPILLAIRE
Abstract
(EN)
Methods are provided for detection and quantitation of mixtures containing target nucleobase sequences using capillary electrophoresis. Peptide nucleic acid oligomers, complementary to the target sequences and preferably having appended detectable labels, are hybridized to the targets. Capillary electrophoresis is then performed, and the detectable label is detected and quantitated.
(FR)
La présente invention concerne le recours à l'électrophorèse capillaire pour la détection et le dosage de mélanges contenant des séquences de nucléobases cibles. Des oligomères peptides d'acides nucléiques, complémentaires des séquences cibles, et présentant de préférence des étiquettes détectables, sont hybridés aux cibles. L'électrophorèse pratiquée ensuite permet de détecter et de faire la numération des étiquettes détectables.
Also published as
Latest bibliographic data on file with the International Bureau