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1. WO1996006186 - NUCLEOTIDIC FRAGMENTS CAPABLE OF HYBRIDIZING SPECIFICALLY TO rDNA OR rRNA OF A RICKETTSIA AND UTILISATION AS PROBES OR INITIATORS

Publication Number WO/1996/006186
Publication Date 29.02.1996
International Application No. PCT/FR1995/001114
International Filing Date 24.08.1995
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/689
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
689for bacteria
Y02A 50/30
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
50in human health protection
30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Applicants
  • BIO MERIEUX [FR]/[FR] (AllExceptUS)
  • MABILAT, Claude [FR]/[FR] (UsOnly)
  • RAOULT, Didier [FR]/[FR] (UsOnly)
Inventors
  • MABILAT, Claude
  • RAOULT, Didier
Agents
  • TONNELLIER, Jean-Claude
Priority Data
94/1026324.08.1994FR
Publication Language French (FR)
Filing Language French (FR)
Designated States
Title
(EN) NUCLEOTIDIC FRAGMENTS CAPABLE OF HYBRIDIZING SPECIFICALLY TO rDNA OR rRNA OF A RICKETTSIA AND UTILISATION AS PROBES OR INITIATORS
(FR) FRAGMENTS NUCLEOTIDIQUES CAPABLES DE S'HYBRIDER SPECIFIQUEMENT A L'ADNr ou ARNr DES RICKETTSIA ET LEUR UTILISATION COMME SONDES OU AMORCES
Abstract
(EN)
The invention relates to nucleotidic monocatenary fragments belonging to preserved or variables regions of ribosomal RNA (16S) of bacteria of the Rickettsia genus. Said fragments are capable of hybridizing in predetermined conditions to rDNA or rRNA of at least one bacteria of the Rickettsia genus and not to rDNA or rRNA of non-Rickettsia. The invention also relates to probes, initiators whose sequences belong to said fragments of the invention, as well as to a reactant and a method for identifying the Rickettsia genus or a species of the Rickettsia genus.
(FR)
L'invention concerne des fragments nucléotidiques monocaténaires appartenant à des régions conservées ou variables de l'ARN ribosomique 16S de bactéries du genre Rickettsia. Ces fragments sont capables de s'hybrider dans des conditions prédéterminées à l'ADNr ou l'ARNr d'au moins une bactérie du genre Rickettsia et non à l'ADNr ou l'ARNr des non-Rickettsia. L'invention concerne en outre les sondes, les amorces dont les séquences appartiennent à celles des fragments de l'invention, ainsi qu'un réactif et un procédé pour identifier le genre Rickettsia, ou une espèce du genre Rickettsia.
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