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1. WO1996006185 - ASSAY FOR THE DETECTION OF PROTEASES

Publication Number WO/1996/006185
Publication Date 29.02.1996
International Application No. PCT/AU1995/000536
International Filing Date 25.08.1995
Chapter 2 Demand Filed 23.02.1996
IPC
A61K 38/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
C07K 5/083 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
5Peptides having up to four amino acids in a fully defined sequence; Derivatives thereof
04containing only normal peptide links
08Tripeptides
083the side chain of the first amino acid being acyclic, e.g. Gly, Ala
C12Q 1/37 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
34involving hydrolase
37involving peptidase or proteinase
CPC
A61K 38/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
C07K 5/0806
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
5Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
04containing only normal peptide links
08Tripeptides
0802with the first amino acid being neutral
0804and aliphatic
0806the side chain containing 0 or 1 carbon atoms, i.e. Gly, Ala
C12Q 1/37
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
34involving hydrolase
37involving peptidase or proteinase
Applicants
  • COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION [AU/AU]; Limestone Avenue Campbell, ACT 2601, AU (AllExceptUS)
  • WHITTAKER, Robert, George [AU/AU]; AU (UsOnly)
  • YU, Yan [CN/AU]; AU (UsOnly)
Inventors
  • WHITTAKER, Robert, George; AU
  • YU, Yan; AU
Agents
  • F.B. RICE & CO.; 28A Montague Street Balmain, NSW 2041, AU
Priority Data
PM 769325.08.1994AU
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) ASSAY FOR THE DETECTION OF PROTEASES
(FR) TECHNIQUE DE DETECTION DE PROTEASES
Abstract
(EN)
The present invention relates to a method of detecting the presence of an active protease in a sample. The method involves the following steps: (1) providing a solid support to which is attached a ligand reactive with a protease; (2) contacting the solid support with a sample suspected to contain the protease; (3) washing the solid support to remove material not bound to the ligand; (4) contacting the washed solid support with a solution containing a peptide ester substrate which is hydrolysed for the protease; and (5) detecting protons produced by hydrolysis of the peptide ester substrate. In addition, the present invention provides a prostate specific antigen substrate, the substrate being of the general formula: A-B-Tyrosine-ester in which A is acetyl or carbobenzoxy and B is 2 to 6 amino acids.
(FR)
L'invention concerne un procédé qui permet de détecter la présence d'une protéase active dans un échantillon. Ce procédé consiste à: (1) prendre un support solide auquel est fixé un ligand pouvant réagir avec une protéase; (2) mettre ce support en contact avec un échantillon soupçonné de contenir la protéase; (3) laver le support solide pour éliminer les éléments non liés au ligand; (4) mettre le support solide lavé en contact avec une solution contenant un substrat à base de peptide-ester qui est hydrolysé en fonction de cette protéase; et (5) détecter des protons produits par l'hydrolyse de ce substrat à base de peptide-ester. L'invention porte aussi sur un substrat contenant un antigène spécifique de la prostate, ce substrat correspondant à la formule générale: A-B-tyrosine-ester, où A représente acétyle ou carbobenzoxy, et B représente 2 à 6 acides aminés.
Also published as
NO19970826
Latest bibliographic data on file with the International Bureau