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1. WO1996006172 - KETO GROUP INTRODUCING ENZYME, DNA CODING FOR THE SAME, AND PROCESS FOR PRODUCING KETOCAROTENOID

Publication Number WO/1996/006172
Publication Date 29.02.1996
International Application No. PCT/JP1995/001640
International Filing Date 18.08.1995
IPC
C12N 9/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
C12P 7/26 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
24containing a carbonyl group
26Ketones
C12P 23/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
23Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
CPC
C12N 9/0004
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
C12P 23/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
23Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
C12P 7/26
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
24containing a carbonyl group
26Ketones
Applicants
  • KIRIN BEER KABUSHIKI KAISHA [JP]/[JP] (AllExceptUS)
  • KAJIWARA, Susumu [JP]/[JP] (UsOnly)
  • MISAWA, Norihiko [JP]/[JP] (UsOnly)
  • KONDO, Keiji [JP]/[JP] (UsOnly)
Inventors
  • KAJIWARA, Susumu
  • MISAWA, Norihiko
  • KONDO, Keiji
Agents
  • HIRAKI, Yusuke
Priority Data
6/19877523.08.1994JP
6/22379819.09.1994JP
7/4726607.03.1995JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) KETO GROUP INTRODUCING ENZYME, DNA CODING FOR THE SAME, AND PROCESS FOR PRODUCING KETOCAROTENOID
(FR) ENZYME D'INTRODUCTION DU GROUPE CETO, ADN CODANT POUR CETTE ENZYME ET PROCEDE D'ELABORATION DE CETOCAROTENOIDE
Abstract
(EN)
A polypeptide having the enzymatic activity of converting the 4-methylene group of a $g(b)-ionone compound into a keto group; a DNA containing the base sequence coding for the above polypeptide; another DNA which hybridizes with the above DNA and contains the base sequence coding for the above polypeptide; still another DNA which has been inserted into plasmid pHP51 and contains the base sequence coding for the above polypeptide; a recombinant vector containing the above DNA(s); a microbe having the above DNA(s) introduced thereinto; and a process for producing a ketocarotenoid which comprises culturing the above microbe in a medium and separating the formed ketocarotenoid from the product of culture. The introduction of the above DNAs as foreign genes into microbes, such as $i(E. coli), followed by expression thereof makes it possible to impart to the microbes the capability of biosynthesis of astaxanthin, 4-ketozeaxanthin, canthaxanthin, echinenone and other ketocarotenoids. The use of such microbes makes it possible to mass-produce ketocarotenoids at reduced cost and labor.
(FR)
Polypeptide exerçant une activité enzymatique de conversion du groupe 4-méthylène du composé $g(b)-ionone en un groupe céto; ADN renfermant la séquence de base codant le polypeptide précité; autre ADN réalisant l'hybridation avec l'ADN précité et contenant la séquence de base codant le polypeptide précité; autre ADN ayant été inséré dans un plasmide pHP51 et comprenant la séquence de base codant le polypeptide précité; vecteur de recombinaison renfermant les ADN précités; microbe dans lequel les ADN précités ont été introduits; et procédé d'élaboration de cétocaroténoïde consistant à cultiver le microbe sus-visé dans un milieu et à séparer la cétocaroténoïde ainsi formée de produits de culture. L'introduction des ADN sus-visés comme gènes étrangers dans des microbes, tels que des $i(E. Coli), suivie par l'expression de ces gènes permet de conférer à ces microbes une aptitude à la biosynthèse de l'astaxanthine, la 4-cétozéaxanthine, la canthaxanthine, l'échinenone et d'autres cétocaroténoïdes. L'utilisation de tels microbes permet de produire en masse des cétocaroténoïdes pour un coût modique.
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