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1. WO1996005317 - GA4 DNA, PROTEIN AND METHODS OF USE

Publication Number WO/1996/005317
Publication Date 22.02.1996
International Application No. PCT/US1995/010403
International Filing Date 15.08.1995
Chapter 2 Demand Filed 15.03.1996
IPC
C12N 9/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
C12N 15/82 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
CPC
C12N 15/8297
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8261with agronomic (input) traits, e.g. crop yield
8291Hormone-influenced development
8297Gibberellins; GA3
C12N 9/0071
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0071acting on paired donors with incorporation of molecular oxygen (1.14)
Applicants
  • THE GENERAL HOSPITAL CORPORATION [US]/[US]
Inventors
  • CHIANG, Hui-Hwa
  • HWANG, Inhwan
  • GOODMAN, Howard, M.
Agents
  • CIMBALA, Michele, A.
Priority Data
08/291,93916.08.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) GA4 DNA, PROTEIN AND METHODS OF USE
(FR) ADN GA4, PROTEINE CODEE ET UTILISATION
Abstract
(EN)
The invention relates to the DNA and protein encoded by the $i(GA4) locus. This protein is believed to be a member of the family of enzymes involved in the biosynthesis of the gibberellin family (GA) of plan growth hormones which promote various growth and developmental processes in higher plants, such as seed germination, stem elongation, flowering and fruiting. More specifically, the protein encoded by the $i(GA4) locus is an hydroxylase. The invention also relates to vectors containing the DNA and the expression of the protein encoded by the DNA of the invention in a host cell. Additional aspects of the invention are drawn to host cells transformed with the DNA or antisense sequence of the invention, the use of such host cells for the maintenance, or expression or inhibition of expression of the DNA of the invention and to transgenic plants containing DNA of the invention. Finally, the invention also relates to the use of the protein encoded by the $i(GA4) locus to alter aspects of plant growth.
(FR)
ADN et protéine codée par le locus de $i(GA4). Cette protéine serait un membre de la famille des enzymes impliqués dans la biosynthèse de la famille gibbérelline (GA) des hormones de croissance des végétaux qui favorisent divers processus de croissance et de développement chez les plantes supérieures, comme la germination des graines, la croissance de la tige, la floraison et la fructification. De manière plus spécifique, la protéine codée par le locus de $i(GA4) est une hydroxylase. L'invention porte également sur des vecteurs renfermant l'ADN et l'expression de la protéine codée par l'ADN visé dans cette invention dans une cellule hôte. Elle porte également sur d'autres aspects tels que des cellules hôtes transformées par l'ADN ou la séquence antisens visée ici, l'utilisation de telles cellules hôtes pour la préservation, l'expression ou l'inhibition de l'expression de cet ADN, et sur des végétaux transgéniques renfermant cet ADN. Enfin, elle porte également sur l'utilisation de la protéine codée par le locus de $i(GA4) pour modifier certains aspects de la croissance des végétaux.
Also published as
NZ291346
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