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1. WO1996005296 - METHOD OF PREPARING AND AMPLIFYING NUCLEIC ACIDS

Publication Number WO/1996/005296
Publication Date 22.02.1996
International Application No. PCT/DE1995/001003
International Filing Date 28.07.1995
Chapter 2 Demand Filed 12.03.1996
IPC
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12N 15/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/6813
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
C12Q 1/686
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
686Polymerase chain reaction [PCR]
Applicants
  • INSTITUT FÜR MOLEKULARE BIOTECHNOLOGIE, E.V. [DE/DE]; Beutenbergstrasse 11 D-07745 Jena, DE (AllExceptUS)
  • GREULICH, Karl-Otto [DE/DE]; DE (UsOnly)
  • CELEDA, Dino [DE/DE]; DE (UsOnly)
Inventors
  • GREULICH, Karl-Otto; DE
  • CELEDA, Dino; DE
Agents
  • PERREY, Ralf; Müller-Boré & Partner Grafinger Strasse 2 D-81671 München, DE
Priority Data
P 44 28 651.112.08.1994DE
Publication Language German (DE)
Filing Language German (DE)
Designated States
Title
(DE) VERFAHREN ZUR HERSTELLUNG UND AMPLIFIKATION VON NUKLEINSÄUREN
(EN) METHOD OF PREPARING AND AMPLIFYING NUCLEIC ACIDS
(FR) PROCEDE DE PREPARATION ET D'AMPLIFICATION D'ACIDES NUCLEIQUES
Abstract
(DE)
Die vorliegende Erfindung betrifft ein Verfahren zur Herstellung und/oder Amplifikation von Nukleinsäuren, deren Verwendung sowie einen die erfindungsgemäß hergestellten Nukleinsäuren enthaltenden Kit. Insbesondere betrifft diese Erfindung ein Verfahren zur Herstellung und/oder Amplifikation von Nukleinsäuresequenzen ohne Zugabe von Startern ('Primer').
(EN)
The invention concerns a method for the preparation and/or amplification of nucleic acids, as well as the use of nucleic acids prepared in this way and a kit containing them. In particular, the invention concerns a method of preparing and/or amplifying nucleic-acid sequences without the addition of so-called primers.
(FR)
L'invention concerne un procédé de préparation et/ou d'amplification d'acides nucléiques, leur utilisation et un nécessaire contenant les acides nucléiques préparés selon l'invention. L'invention concerne notamment un procédé de préparation et/ou d'amplification de séquences d'acides nucléiques sans adjonction d'amorces.
Also published as
Latest bibliographic data on file with the International Bureau