Processing

Please wait...

Settings

Settings

1. WO1996004405 - NOVEL DIMERIC FLUORESCENT ENERGY TRANSFER DYES COMPRISING ASYMMETRIC CYANINE AZOLE-INDOLENINE CHROMOPHORES

Publication Number WO/1996/004405
Publication Date 15.02.1996
International Application No. PCT/US1995/009987
International Filing Date 26.07.1995
Chapter 2 Demand Filed 05.02.1996
IPC
C07D 417/14 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
DHETEROCYCLIC COMPOUNDS
417Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/196
14containing three or more hetero rings
C09B 23/02 2006.01
CCHEMISTRY; METALLURGY
09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES; MORDANTS; LAKES
23Methine or polymethine dyes, e.g. cyanine dyes
01characterised by the methine chain
02containing an odd number of CH groups
G01N 27/447 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
CPC
C07D 417/14
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
DHETEROCYCLIC COMPOUNDS
417Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
14containing three or more hetero rings
C09B 23/02
CCHEMISTRY; METALLURGY
09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES ; , e.g. PIGMENTS; MORDANTS; LAKES
23Methine or polymethine dyes, e.g. cyanine dyes
02the polymethine chain containing an odd number of >CH- ; or >C[alkyl]-; groups
G01N 27/44726
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
44704Details; Accessories
44717Arrangements for investigating the separated zones, e.g. localising zones
44721by optical means
44726using specific dyes, markers or binding molecules
Applicants
  • THE REGENTS OF THE UNIVERSITY OF CALIFORNIA [US/US]; 22nd floor 300 Lakeside Drive Oakland, CA 94612-3550, US
Inventors
  • GLAZER, Alexander, N.; US
  • BENSON, Scott, C.; US
Agents
  • ROWLAND, Bertram, I. ; Flehr, Hohbach, Test, Albritton & Herbert Suite 3400 4 Embarcadero Center San Francisco, CA 94111-4187, US
Priority Data
08/283,00629.07.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) NOVEL DIMERIC FLUORESCENT ENERGY TRANSFER DYES COMPRISING ASYMMETRIC CYANINE AZOLE-INDOLENINE CHROMOPHORES
(FR) NOUVEAUX COLORANTS DIMERES FLUORESCENTS TRANSFERANT L'ENERGIE COMPRENANT DES CHROMOPHORES ASYMETRIQUES CYANINE AZOLE INDOLENINE
Abstract
(EN)
Novel fluorescent heterodimeric DNA-staining energy transfer dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts. Kits and individual compounds are provided, where the kits find use for simultaneous detection of a variety of moieties, particularly using a single narrow wavelength irradiation source. The individual compounds are characterized by high donor quenching and high affinity to dsDNA as a result of optimizing the length of the linking group separating the two chromophores.
(FR)
De nouveaux colorants hétérodimères fluorescents qui transfèrent l'énergie et colorent l'ADN combinent des colorants asymétriques cyanine azole indolénine et présentent une forte affinité pour l'ADN, de grands décalages Stokes et une émission dans la région rouge du spectre. Ces colorants trouvent une application particulière dans l'électro-phorèse sur gel et pour produire des étiquettes qui peuvent être liées à diverses compositions dans des contextes variés. Des kits et des composés individuels sont décrits, les kits pouvant être utilisés pour détecter simultanément des fractions différentes, plus particulièrement à l'aide d'une source d'irradiation unique à longueur d'onde étroite. Les composés individuels se caractérisent par une forte extinction du donneur et une forte affinité pour l'ADN bicaténaire qui résultent de l'optimisation de la longueur du groupe de liaison séparant les deux chromophores.
Latest bibliographic data on file with the International Bureau