Processing

Please wait...

Settings

Settings

1. WO1996004401 - METHOD AND DEVICE FOR MEASURING THE CONCENTRATION OF A SUBSTANCE IN A SOLUTION

Publication Number WO/1996/004401
Publication Date 15.02.1996
International Application No. PCT/SE1995/000888
International Filing Date 28.07.1995
Chapter 2 Demand Filed 22.11.1995
IPC
C12Q 1/58 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
58involving urea or urease
G01N 27/02 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
02by investigating impedance
CPC
C12Q 1/58
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
58involving urea or urease
G01N 27/021
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
02by investigating the impedance of the material
021before and after chemical transformation of the material
G01N 33/493
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
483Physical analysis of biological material
487of liquid biological material
493urine
Y10T 436/171538
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
436Chemistry: analytical and immunological testing
17Nitrogen containing
171538Urea or blood urea nitrogen
Y10T 436/25
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
436Chemistry: analytical and immunological testing
25including sample preparation
Applicants
  • GAMBRO AB [SE/SE]; P.O. Box 10101 S-220 10 Lund, SE (AllExceptUS)
  • EDGSON, Raymond [GB/GB]; GB (UsOnly)
  • OLDE, Bo [SE/SE]; SE (UsOnly)
  • OLSSON, Lars-Fride [SE/SE]; SE (UsOnly)
  • GOODMAN JONES, Arfon [GB/GB]; GB (UsOnly)
Inventors
  • EDGSON, Raymond; GB
  • OLDE, Bo; SE
  • OLSSON, Lars-Fride; SE
  • GOODMAN JONES, Arfon; GB
Agents
  • ASKETORP, Göran; Gambro AB P.O. Box 10101 S-220 10 Lund, SE
Priority Data
9402602-829.07.1994SE
9402835-424.08.1994SE
9501315-710.04.1995SE
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD AND DEVICE FOR MEASURING THE CONCENTRATION OF A SUBSTANCE IN A SOLUTION
(FR) METHODE ET DISPOSITIF DE MESURE DE LA CONCENTRATION D'UNE SUBSTANCE DANS UNE SOLUTION
Abstract
(EN)
Method and device for measuring the concentration of urea or a similar substance, in a composite solution, particularly a dialysis solution, by catalytic decomposition of urea by urease. The differential conductivity of the reacted and unreacted solution is measured and is proportional to the urea concentration. The decomposition occurs according to the reaction: (NH2)2 CO + CO2 + 3H2O $m(7) 2NH4+ + 2HCO3- (5). By the addition (41, 60) of carbon dioxide gas and by catalyzation with the enzyme urease, the reaction is displaced to the right, which means that said proportionality is valid over a large range of urea concentrations, up to and exceeding 35 mM. The addition of carbon dioxide gas also ensures sufficiently low pH-value in the solution in order to avoid precipitation of calcium carbonate.
(FR)
Méthode et dispositif de mesure de la concentration de l'urée ou d'une substance similaire, dans une solution composite, notamment une solution de dialyse, par décomposition catalytique de l'urée par l'uréase. On mesure la différence de conductivité entre la solution ayant subi la réaction et la solution ne l'ayant pas subi, cette conductivité étant proportionnelle à la concentration d'urée. La décomposition s'opère selon la réaction suivante: (NH2)2 CO + CO2 + 3H2O $m(7) 2NH4+ + 2HCO3- (5). Sous l'effet de l'addition (41, 60) de dioxide de carbone sous forme gazeuse et de la catalysation par l'uréase, la réaction se déplace vers la droite, ce qui signifie que cette proportionalité s'applique sur une vaste plage de concentrations d'urée, jusqu'à et même au-delà de 35 mM. L'addition de dioxide de carbone sous forme gazeuse assure également une valeur de pH suffisamment basse dans la solution pour prévenir la précipitation de carbonate de calcium.
Latest bibliographic data on file with the International Bureau