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1. WO1996004390 - DNA SEQUENCE WHICH ACTS AS A CHROMATIN INSULATOR ELEMENT TO PROTECT EXPRESSED GENES FROM CIS-ACTING REGULATORY SEQUENCES IN MAMMALIAN CELLS

Publication Number WO/1996/004390
Publication Date 15.02.1996
International Application No. PCT/US1995/009473
International Filing Date 28.07.1995
Chapter 2 Demand Filed 07.02.1996
IPC
C07K 14/465 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
465from birds
C12N 15/63 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N 15/85 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
CPC
C07K 14/465
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
465from birds
C12N 15/63
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N 15/85
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
C12N 2800/90
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2800Nucleic acids vectors
90Vectors containing a transposable element
C12N 2830/008
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2830Vector systems having a special element relevant for transcription
008cell type or tissue specific enhancer/promoter combination
C12N 2830/40
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2830Vector systems having a special element relevant for transcription
40being an insulator
Applicants
  • THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES [US]/[US]
Inventors
  • CHUNG, Jay, H.
  • FELSENFELD, Gary
Agents
  • FEILER, William, S.
Priority Data
08/283,12529.07.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) DNA SEQUENCE WHICH ACTS AS A CHROMATIN INSULATOR ELEMENT TO PROTECT EXPRESSED GENES FROM CIS-ACTING REGULATORY SEQUENCES IN MAMMALIAN CELLS
(FR) SEQUENCE D'ADN FONCTIONNANT COMME ELEMENT ISOLANT DE LA CHROMATINE PERMETTANT DE PROTEGER DES GENES EXPRIMES CONTRE DES SEQUENCES REGULATRICES A FONCTIONNEMENT $i(CIS) DANS LES CELLULES DE MAMMIFERES
Abstract
(EN)
A newly characterized chromatin insulator element isolated from the DNA of a vertebrate organism and contained in vector constructs is described. The insulator element of the invention comprises a DNA sequence which contains a 5' constitutive hypersensitive site and whose functional activity and biochemical characterization as a pure insulator were previously unknown. Such a DNA molecule having insulating function has been isolated from the $g(b)-globin gene locus and from the $g(a)-globin gene locus of chicken. A core DNA sequence having strong insulator activity is also described. The insulator elements, including the core sequence, have been demonstrated for the first time in mammalian cells to function to buffer or insulate an expressed gene from the activity of $i(cis)-acting regulatory elements, such as enhancers, in the surrounding chromatin or DNA.
(FR)
La présente invention concerne un élément nouvellement caractérisé fonctionnant comme isolant de la chromatine. Il a été isolé de l'ADN d'un organisme de vertébré et se trouve dans des constructions de vecteurs. L'élément isolant de l'invention comporte une séquence ADN qui contient un site hypersensible constitutif de 5' et dont l'activité fonctionnelle et les caractéristiques biochimiques en tant que pur isolant étaient inconnues jusqu'alors. Ce type de molécule d'ADN présentant une fonction isolante a été isolé à partir du locus du gène de la bêta-globine, et à partir du locus du gène de l'alpha-globine des poulets. L'invention concerne également une séquence d'ADN noyau faisant preuve d'une puissante activité isolante. Il a été démontré pour la première fois dans les cellules de mammifère que les éléments isolants, y compris la séquence noyau, ont une fonction de tampon ou d'isolant d'un gène exprimé par rapport aux éléments régulateurs à fonctionnement $i(cis), tels que les amplificateurs, dans la chromatine environnante ou l'ADN.
Also published as
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