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1. WO1996003654 - METHOD OF IMMUNOASSAYING AUTOANTIBODY

Publication Number WO/1996/003654
Publication Date 08.02.1996
International Application No. PCT/JP1995/001466
International Filing Date 24.07.1995
IPC
G01N 33/564 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
564for pre-existing immune complex or autoimmune disease
CPC
G01N 2800/104
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2800Detection or diagnosis of diseases
10Musculoskeletal or connective tissue disorders
101Diffuse connective tissue disease, e.g. Sjögren, Wegener's granulomatosis
104Lupus erythematosus [SLE]
G01N 2800/24
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2800Detection or diagnosis of diseases
24Immunology or allergic disorders
G01N 33/564
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
564for pre-existing immune complex or autoimmune disease ; , i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
Applicants
  • KANEGAFUCHI KAGAKU KOGYO KABUSHIKI KAISHA [JP/JP]; 2-4, Nakanoshima 3-chome Kita-ku Osaka-shi Osaka 530, JP (AllExceptUS)
  • YABUKI, Tetsuro [JP/JP]; JP (UsOnly)
Inventors
  • YABUKI, Tetsuro; JP
Agents
  • YAMAMOTO, Shusaku; Crystal Tower, 15th floor 2-27, Shiromi 1-chome Chuo-ku, Osaka-shi Osaka 540, JP
Priority Data
6/17272625.07.1994JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) METHOD OF IMMUNOASSAYING AUTOANTIBODY
(FR) PROCEDE DE DOSAGE IMMUNOLOGIQUE D'AUTO-ANTICORPS
Abstract
(EN)
A method of immunoassaying an antibody characteristic of systemic lupus erythematosus (SLE) and a kit therefor. The quantity of an antibody which combines with a negatively charged substance by means of electrostatic affinity can be obtained by reacting a body fluid with the substance in each of at least three buffers having different ionic strengths, measuring the quantity of the antibody combined with the substance in each buffer, and determining the difference between the minimum value of the measured quantity of the combined antibody and the maximum value of the measured quantity thereof on the lower ionic-strength side. Thus it is possible to assay specifically and easily the autoantibody that combines with an antigen by means of electrostatic affinity and that is thought to relate seriously to the pathological condition of SLE, and to diagnose SLE more accurately.
(FR)
Procédé servant à réaliser le dosage immunologique d'un anticorps caractéristique du lupus érythémateux aigu disséminé (SLE) et kit de mise en application dudit procédé. On peut obtenir la quantité d'un anticorps se combinant avec une substance chargée négativement au moyen d'une affinité électrostatique par réaction d'un fluide corporel avec ladite substance dans chacun de trois tampons au moins possédant des forces ioniques différentes, par mesure de la quantité de l'anticorps combiné avec la substance dans chaque tampon et par détermination de la différence entre la valeur minimum de la quantité mesurée de l'anticorps combiné et la valeur maximum de la quantité mesurée dudit anticorps du côté inférieur de force ionique. Il est, de ce fait, possible de réaliser facilement un dosage spécifique de l'auto-anticorps se combinant avec un antigène au moyen d'une affinité électrostatique et vraisemblablement impliqué dans l'état pathologique de SLE, ainsi que de diagnostiquer SLE avec davantage de précision.
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