Processing

Please wait...

PATENTSCOPE will be unavailable a few hours for maintenance reason on Saturday 31.10.2020 at 7:00 AM CET
Settings

Settings

Goto Application

1. WO1996003637 - DETERMINATION OF LIGHT ABSORPTION PATHLENGTH IN A VERTICAL-BEAM PHOTOMETER

Publication Number WO/1996/003637
Publication Date 08.02.1996
International Application No. PCT/US1995/009459
International Filing Date 25.07.1995
Chapter 2 Demand Filed 23.02.1996
IPC
G01N 21/25 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
G01N 21/31 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
CPC
G01N 21/253
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
251Colorimeters; Construction thereof
253for batch operation, i.e. multisample apparatus
G01N 21/3151
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
314with comparison of measurements at specific and non-specific wavelengths
3151using two sources of radiation of different wavelengths
Applicants
  • MOLECULAR DEVICES CORPORATION [US]/[US]
Inventors
  • HAFEMAN, Dean, G.
Agents
  • DREHKOFF, W., Dennis
Priority Data
08/279,51825.07.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) DETERMINATION OF LIGHT ABSORPTION PATHLENGTH IN A VERTICAL-BEAM PHOTOMETER
(FR) DETERMINATION DE LA LONGUEUR DE TRAJET D'ABSORPTION LUMINEUSE DANS UN PHOTOMETRE A FAISCEAU VERTICAL
Abstract
(EN)
Disclosed are photometric methods and devices for determining optical pathlength of liquid samples containing analytes dissolved or suspended in a solvent. The methods and devices rely on determining a relationship between the light absorption properties of the solvent and the optical pathlength of liquid samples containing the solvent. This relationship is used to establish the optical pathlength for samples containing an unknown concentration of analyte but having similar solvent composition. Further disclosed are methods and devices for determining the concentration of analyte in such samples where both the optical pathlength and the concentration of analyte are unknown. The methods and devices rely on separately determining, at different wavelengths of light, light absorption by the solvent and light absorption by the analyte. Light absorption by the analyte, together with the optical pathlength so determined, is used to calculate the concentration of the analyte. Devices for carrying out the methods particularly advantageously include vertical-beam photometers containing samples disposed within the wells of multi-assays plates, wherein the photometer is able to monitor light absorption of each sample at multiple wavelengths, including in the visible or UV-visible region of the spectrum, as well as in the near-infrared region of the electromagnetic spectrum. Novel photometer devices are described which automatically determine the concentration of analytes in such multi-assay plates directly without employing a standard curve.
(FR)
La présente invention concerne des procédés et dispositifs de détermination de la longueur de trajet optique d'échantillons liquides contenant des analytes en solution ou en suspension dans un solvant. Le principe mis en ÷uvre tant pour les procédés que pour les dispositifs repose sur la détermination d'une relation entre les propriétés d'absorption de la lumière par le solvant et la longueur de trajet optique des échantillons liquides contenant le solvant. Cette relation sert à déterminer la longueur de trajet optique pour des échantillons contenant une concentration inconnue de l'analyte pour une composition similaire de solvant. L'invention concerne également des procédés et dispositifs de détermination de la concentration de l'analyte dans de tels échantillons pour lesquels on ne connaît ni la longueur de trajet optique ni la concentration de l'analyte. Le principe mis en ÷uvre pour ces procédés et dispositifs repose sur une détermination séparée, à différentes longueurs d'ondes lumineuses, de l'absorption lumineuse par le solvant et de l'absorption lumineuse par l'analyte. L'absorption lumineuse par ce dernier et la longueur de trajet optique ainsi déterminée, servent ensemble à calculer la concentration de l'analyte. Les dispositifs de mise en ÷uvre des procédés sont avantageusement réalisés au moyen de photomètres à faisceau vertical où les échantillons sont disposés à l'intérieur de puits de plaques de multititrage, pour que le photomètre puisse mesurer l'absorption lumineuse par chaque échantillon, à différentes longueurs d'ondes, y compris dans la région visible ou dans les régions U.V. et visible du spectre, de même que dans la région infrarouge proche du spectre électromagnétique. L'invention concerne en outre de nouveaux dispositifs de photomètres déterminant automatiquement dans des plaques de multititrage la concentration d'un analyte, directement sans avoir recours à une courbe d'étalonnage.
Also published as
Latest bibliographic data on file with the International Bureau