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1. WO1996003632 - METHOD FOR PRESERVING CELLS, AND USES OF SAID METHOD

Publication Number WO/1996/003632
Publication Date 08.02.1996
International Application No. PCT/US1995/009653
International Filing Date 27.07.1995
Chapter 2 Demand Filed 27.02.1996
IPC
G01N 1/30 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
30Staining; Impregnating
G01N 33/50 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G01N 33/569 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
G01N 33/574 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
574for cancer
CPC
G01N 1/30
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
G01N 33/5005
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
5005involving human or animal cells
G01N 33/56966
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
56966Animal cells
G01N 33/57492
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
574for cancer
57484involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
57492involving compounds localized on the membrane of tumor or cancer cells
Y10S 435/96
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
435Chemistry: molecular biology and microbiology
96Immunohistochemical assay
Y10S 435/962
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
435Chemistry: molecular biology and microbiology
962Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
Applicants
  • THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK [US/US]; West 116th Street and Broadway New York, NY 10027, US
Inventors
  • TSE, Doris, B.; US
  • CHUNG, Hui-Min; US
  • VAN DER PLOEG, Lex; US
Agents
  • WHITE, John, P.; Cooper & Dunham LLP 1185 Avenue of the Americas New York, NY 10036, US
Priority Data
08/281,46027.07.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD FOR PRESERVING CELLS, AND USES OF SAID METHOD
(FR) PROCEDE DE CONSERVATION DE CELLULES, ET UTILISATIONS DE CE PROCEDE
Abstract
(EN)
The invention provides a method for preserving cells which comprises (a) suspending cells in a physiologic isotonic medium; and (b) fixing the cells so suspended at a temperature of less than about 10 degrees C under sufficiently hypertonic conditions to disperse the cells in a single, unagglutinated state. Also provided are methods for separating such preserved cells from a sample in order to detect, quantitate and/or visualize the preserved cells, their metabolic processes and their surface receptors. Such preserved cells can be assayed for phenotype as well as the presence of malignancy. Fetal cells preserved by the described method can be assayed to determine gender or presence of fetal abnormalities.
(FR)
La présente invention concerne un procédé pour conserver des cellules qui consiste (a) à placer les cellules en suspension dans un milieu isotonique, physiologique; et (b) fixer les cellules en suspension, à une température inférieure à environ 10 degrés C dans des conditions hypertoniques suffisantes pour disperser les cellules dans un état unitaire, non agglutiné. L'invention traite également de procédés pour séparer ces cellules ainsi conservées d'un échantillon afin de détecter, quantifier et/ou visualiser les cellules conservées, leurs processus métaboliques et leurs récepteurs de surface. On peut analyser ces cellules conservées afin de détecter leur phénotype ou la présence éventuelle d'une malignité. On peut analyser les cellules foetales conservées par le procédé décrit pour déterminer le genre ou la présence d'anomalies foetales.
Also published as
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