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1. WO1996003526 - NUCLEIC ACID AMPLIFICATION METHOD USING A MODIFIED NUCLEOSIDE, AND DETECTION OF THE AMPLIFICATION PRODUCT USING ANTIBODIES

Publication Number WO/1996/003526
Publication Date 08.02.1996
International Application No. PCT/FR1995/000985
International Filing Date 21.07.1995
Chapter 2 Demand Filed 26.01.1996
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/6804
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6804Nucleic acid analysis using immunogens
C12Q 1/686
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
686Polymerase chain reaction [PCR]
Applicants
  • PARTEUROP DEVELOPPEMENT [FR/FR]; 32, avenue Félix-Faure F-69007 Lyon, FR (AllExceptUS)
  • NIVELEAU, Alain [FR/FR]; FR (UsOnly)
Inventors
  • NIVELEAU, Alain; FR
Agents
  • TONNELLIER, Jean-Claude; Nony & Associés 29, rue Cambacérès F-75008 Paris, FR
Priority Data
94/0904121.07.1994FR
Publication Language French (FR)
Filing Language French (FR)
Designated States
Title
(EN) NUCLEIC ACID AMPLIFICATION METHOD USING A MODIFIED NUCLEOSIDE, AND DETECTION OF THE AMPLIFICATION PRODUCT USING ANTIBODIES
(FR) PROCEDE D'AMPLIFICATION D'ACIDE NUCLEIQUE A L'AIDE D'UN NUCLEOSIDE MODIFIE, ET DETECTION DU PRODUIT D'AMPLIFICATION A L'AIDE D'ANTICORPS
Abstract
(EN)
A nucleotide sequence amplification method wherein a polynucleotide containing the sequence is contacted with oligonucleotide primers, nucleoside triphosphates and/or deoxynucleoside triphosphates containing the various purine and pyrimidine bases needed to synthesise the amplification product, and an enzyme system having DNA polymerase and/or RNA polymerase and/or reverse transcriptase activity, under conditions enabling the sequence of interest to be amplified in a $i(per se) known manner. One of the nucleoside triphosphates and/or deoxynucleoside triphosphates is present either in a modified form or in both a modified form and an unmodified form, and, in said modified form, the purine or pyrimidine base is substituted by at least one methyl, hydroxymethyl or acetyl grouping.
(FR)
Procédé d'amplification d'une séquence nucléotidique, dans lequel on met en contact un polynucléotide contenant ladite séquence, des amorces oligonucléotidiques, les nucléosides triphosphates et/ou désoxynucléosides triphosphates contenant les diverses bases puriques et pyrimidiques nécessaires à la synthèse du produit d'amplification, et un système enzymatique ayant une activité de DNA polymérase et/ou de RNA polymérase et/ou de transcriptase inverse, dans des conditions permettant d'effectuer, de façon connue en soi, une amplification de ladite séquence d'intérêt, caractérisé par le fait que l'un desdits nucléosides triphosphates et/ou désoxynucléosides triphosphates est présent soit sous une forme modifiée, soit à la fois sous une forme modifiée et sous une forme non modifiée, et que, dans ladite forme modifiée, la base pyrimidique ou purique est substituée par au moins un groupement méthyle, hydroxyméthyle ou acétyle.
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