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1. WO1996003520 - METHODS FOR THE PREPARATION OF RETROVIRAL PARTICLES AND CELL LINES DEFICIENT IN THE ALPHA-GALACTOSYL EPITOPE

Publication Number WO/1996/003520
Publication Date 08.02.1996
International Application No. PCT/US1995/008920
International Filing Date 14.07.1995
Chapter 2 Demand Filed 20.02.1996
IPC
C07K 16/10 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins, e.g. monoclonal or polyclonal antibodies
08against material from viruses
10from RNA viruses
C07K 16/18 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins, e.g. monoclonal or polyclonal antibodies
18against material from animals or humans
C12N 15/867 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
867Retroviral vectors
CPC
C07K 16/1036
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
08against material from viruses
10from RNA viruses ; , e.g. hepatitis E virus
1036Retroviridae, e.g. leukemia viruses
C07K 16/18
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
18against material from animals or humans
C12N 15/86
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
C12N 2740/13043
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2740Reverse Transcribing RNA Viruses
00011Reverse Transcribing RNA Viruses
10011Retroviridae
13011Gammaretrovirus, e.g. murine leukeamia virus
13041Use of virus, viral particle or viral elements as a vector
13043viral genome or elements thereof as genetic vector
C12N 2740/13051
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2740Reverse Transcribing RNA Viruses
00011Reverse Transcribing RNA Viruses
10011Retroviridae
13011Gammaretrovirus, e.g. murine leukeamia virus
13051Methods of production or purification of viral material
Applicants
  • ALEXION PHARMACEUTICALS, INC. [US/US]; Suite 360, 25 Science Park New Haven, CT 06511, US (AllExceptUS)
  • ROTHER, Russell, P. [US/US]; US (UsOnly)
  • ROLLINS, Scott, A. [US/US]; US (UsOnly)
  • FODOR, William, L. [US/US]; US (UsOnly)
  • SPRINGHORN, Jeremy, P. [US/US]; US (UsOnly)
  • SQUINTO, Stephen, P. [US/US]; US (UsOnly)
Inventors
  • ROTHER, Russell, P.; US
  • ROLLINS, Scott, A.; US
  • FODOR, William, L.; US
  • SPRINGHORN, Jeremy, P.; US
  • SQUINTO, Stephen, P.; US
Agents
  • KLEE, Maurice, M.; 1951 Burr Street Fairfield, CT 06430, US
Priority Data
08/278,63921.07.1994US
08/399,41606.03.1995US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR THE PREPARATION OF RETROVIRAL PARTICLES AND CELL LINES DEFICIENT IN THE ALPHA-GALACTOSYL EPITOPE
(FR) PROCEDE DE PREPARATION DE PARTICULES RETROVIRALES ET DE LIGNEES CELLULAIRES DEFICIENTES EN EPITOPES PROPRES A L'ALPHA-GALACTOSYLE
Abstract
(EN)
Methods and compositions are provided for facilitating gene therapy procedures involving the transduction of target cells with retroviral vector particles in the presence of complement containing body fluids. The reduction of levels of galactose alpha (1, 3) galactosyl epitopes on the retroviral vector particles and/or the blockade of antibody binding to such epitopes have been found to render the particles less sensitive to inactivation by complement mediated mechanisms, and to thus allow transduction in the presence of complement containing body fluids. Means are provided for obtaining such reductions.
(FR)
On décrit des procédés et compositions destinés à faciliter des procédures de thérapie génique et impliquant la transduction de cellules cibles avec des particules de vecteurs retroviraux en présence de fluides corporels contenant des compléments. La réduction des niveaux d'épitopes de galactose alpha (1, 3) galactosyle présents sur ces particules de vecteurs rétroviraux et/ou le blocage de la liaison d'anticorps à ces épitopes a permis de constater une moindre sensibilité de ces particules à une inactivation par des mécanismes de médiation par ces compléments, ce qui permet une transduction en présence de fluides corporels, contenant de tels compléments. On décrit des procédures qui donnent de telles réductions.
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