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1. WO1996003510 - A POLYNUCLEOTIDE HERPES VIRUS VACCINE

Publication Number WO/1996/003510
Publication Date 08.02.1996
International Application No. PCT/US1995/009057
International Filing Date 18.07.1995
Chapter 2 Demand Filed 02.01.1996
IPC
C07K 14/035 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
01DNA viruses
03Herpetoviridae, e.g. pseudorabies virus
035Herpes simplex virus I or II
CPC
A61K 2039/51
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
51comprising whole cells, viruses or DNA/RNA
C07K 14/005
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
C12N 2710/16622
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
16011Herpesviridae
16611Simplexvirus, e.g. human herpesvirus 1, 2
16622New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Applicants
  • MERCK & CO., INC. [US/US]; 126 East Lincoln Avenue Rahway, NJ 07065, US (AllExceptUS)
  • ARMSTRONG, Marcy, E. [US/US]; US (UsOnly)
  • KEYS, Robert, D. [US/US]; US (UsOnly)
  • LEWIS, John, A. [US/US]; US (UsOnly)
  • LIU, Margaret, A. [US/US]; US (UsOnly)
  • McCLEMENTS, William, L. [US/US]; US (UsOnly)
Inventors
  • ARMSTRONG, Marcy, E.; US
  • KEYS, Robert, D.; US
  • LEWIS, John, A.; US
  • LIU, Margaret, A.; US
  • McCLEMENTS, William, L.; US
Common Representative
  • MERCK & CO., INC.; 126 East Lincoln Avenue Rahway, NJ 07065, US
Priority Data
279,45922.07.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) A POLYNUCLEOTIDE HERPES VIRUS VACCINE
(FR) VACCIN POLYNUCLEOTIDIQUE CONTRE LE VIRUS DE L'HERPES
Abstract
(EN)
Genes encoding herpes simplex virus type 2 (HSV-2) proteins were cloned into eukaryotic expression vectors to express the encoded proteins in mammalian muscle cells $i(in vivo). Animals were immunized by injection of these DNA constructs, termed polynucleotide vaccines or PNV, into their muscles. In a DNA titration, it was found that a single immunization of » 0.5 $g(m)g of (one) PNV, gave > 90 % seroconversion by ten weeks post immunization. Immune antisera neutralized both HSV-2 and HSV-1 in cell culture. When animals were challenged with HSV-2, significant (p < .001) protection from lethal infection was achieved following PNV vaccination.
(FR)
On a cloné des gènes codant les protéines du virus du type 2 de l'herpès (VHS-2) dans des vecteurs d'expression eucaryotiques, pour exprimer les protéines codées dans des cellules musculaires de mammifères $i(in vivo.) Des animaux ont été immunisés par l'injection dans leurs muscles de ces produits de recombinaison d'ADN, dénommés vaccins polynucléotidiques (VPN). Un titrage d'ADN a montré qu'une seule immunisation avec » 0,5 $g(m)g de (un) VPN aboutit à une séroconversion supérieure à 90 %, dix semaines après l'immunisation. Des immun-antisérums ont neutralisé aussi bien le VHS-2 que le VHS-1 en culture cellulaire. Lorsqu'on a administré un VHS-2 à des animaux, on a observé une protection significative (p < 0,001) contre une infection létale, après la vaccination avec le VPN.
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