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1. WO1996003498 - PURIFIED HERPES SIMPLEX VIRUS PROTEASE AND METHODS OF PURIFICATION

Publication Number WO/1996/003498
Publication Date 08.02.1996
International Application No. PCT/US1995/009052
International Filing Date 17.07.1995
Chapter 2 Demand Filed 21.02.1996
IPC
C12N 9/50 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
48acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
50Proteinases
CPC
C12N 9/503
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
48acting on peptide bonds (3.4)
50Proteinases ; , e.g. Endopeptidases (3.4.21-3.4.25)
503derived from viruses
Y10S 435/815
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
435Chemistry: molecular biology and microbiology
814Enzyme separation or purification
815by sorption
Applicants
  • MERCK & CO., INC. [US/US]; 126 East Lincoln Avenue Rahway, NJ 07065, US (AllExceptUS)
  • DARKE, Paul, L. [US/US]; US (UsOnly)
  • HALL, Dawn, L. [US/US]; US (UsOnly)
  • KUO, Lawrence, C. [US/US]; US (UsOnly)
Inventors
  • DARKE, Paul, L.; US
  • HALL, Dawn, L.; US
  • KUO, Lawrence, C.; US
Agents
  • MERCK & CO,. INC; 126 East Lincoln Avenue Rahway NJ 07065, US
Common Representative
  • MERCK & CO., INC.; 126 East Lincoln Avenue Rahway, NJ 07065, US
Priority Data
279,75421.07.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PURIFIED HERPES SIMPLEX VIRUS PROTEASE AND METHODS OF PURIFICATION
(FR) PROTEASE PURIFIEE DU VIRUS DE L'HERPES SIMPLEX ET PROCEDES DE PURIFICATION
Abstract
(EN)
A method of purifying proteases from the Herpes Simplex Virus 1 and 2 is taught. A precursor protease is cloned in a host cell and then separated from the cell culture using cation exchange chromatography. The resulting protease is subjected to autoprocessing conditions and an autolytic cleavage occurs, resulting in mature protease.
(FR)
L'invention concerne un procédé pour purifier les protéases du virus de l'herpès Simplex 1 et 3. On clone un précurseur de protéase dans une cellule hôte et on le sépare de la culture de cellules en utilisant la chromatographie d'échange cationique. La protéase résultante est soumise à des conditions d'auto-traitement pour produire une scission autolytique, conduisant à la protéase définitive.
Also published as
Latest bibliographic data on file with the International Bureau