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1. WO1996003496 - CULTURE MEDIUM PROMOTING ENDOCRINE CELL GROWTH

Publication Number WO/1996/003496
Publication Date 08.02.1996
International Application No. PCT/FR1995/000961
International Filing Date 18.07.1995
IPC
C12N 5/071 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
C12N 5/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
02Propagation of single cells or cells in suspension; Maintenance thereof; Culture media therefor
CPC
C12N 2500/25
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2500Specific components of cell culture medium
05Inorganic components
10Metals; Metal chelators
20Transition metals
24Iron; Fe chelators; Transferrin
25Insulin-transferrin; Insulin-transferrin-selenium
C12N 2500/32
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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2500Specific components of cell culture medium
30Organic components
32Amino acids
C12N 2500/84
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2500Specific components of cell culture medium
70Undefined extracts
80from animals
84from mammals
C12N 2501/01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
01Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
C12N 2501/39
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
30Hormones
38with nuclear receptors
39Steroid hormones
C12N 2501/395
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
30Hormones
38with nuclear receptors
395Thyroid hormones
Applicants
  • DE LEOBARDY, Francis [FR/FR]; FR (AllExceptUS)
  • DUGAST, Hervé [FR/FR]; FR (AllExceptUS)
  • ZABOT, Marie, Thérèse [FR/FR]; FR
  • ZABOT, Jean, Michel [FR/FR]; FR
Inventors
  • ZABOT, Marie, Thérèse; FR
  • ZABOT, Jean, Michel; FR
Common Representative
  • DE LEOBARDY, Francis; Texonniéras F-87270 Couzeix, FR
Priority Data
94/0933022.07.1994FR
Publication Language French (FR)
Filing Language French (FR)
Designated States
Title
(EN) CULTURE MEDIUM PROMOTING ENDOCRINE CELL GROWTH
(FR) MILIEU DE CULTURE FAVORISANT LA CROISSANCE CELLULAIRE DES CELLULES ENDOCRINES
Abstract
(EN)
A culture medium essentially consisting of a combination of a basic nutritive mixture in which the amino acid valine is present in racemic form D, and a dialysed foetal calf serum complement, as well as at least one epidermal growth factor, cholera toxin and hormones. The culture medium may be used for culturing certain endocrine cells capable of incorporating and decarboxylating bioamine precursors. The resulting cells may be used as organ substitutes in transplants.
(FR)
Ce milieu de culture est essentiellement constitué de l'association d'un mélange nutritionnel de base dans lequel l'acide aminé Valine est apporté sous la forme racémique D et d'un complément en sérum de veau f÷tal dialysé, additionné d'au moins un facteur de croissance épidermique, de toxine cholérique et d'hormones. Il s'applique à la culture cellulaire de certaines cellules endocrines capables d'incorporer et de décarboxyler les précurseurs des amines biogènes. Les cellules obtenues par culture sur ce milieu peuvent s'appliquer au greffage comme substitut d'organe.
Also published as
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