Processing

Please wait...

Settings

Settings

1. WO1996003417 - IMPROVED METHODS OF DETRITYLATION FOR OLIGONUCLEOTIDE SYNTHESIS

Publication Number WO/1996/003417
Publication Date 08.02.1996
International Application No. PCT/US1995/009322
International Filing Date 24.07.1995
Chapter 2 Demand Filed 19.02.1996
IPC
C07H 21/00 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
CPC
C07H 21/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
Applicants
  • HYBRIDON, INC. [US/US]; One Innovation Drive Worcester, MA 01605, US (AllExceptUS)
  • HABUS, Ivan [HR/US]; US (UsOnly)
  • AGRAWAL, Sudhir [IN/US]; US (UsOnly)
Inventors
  • HABUS, Ivan; US
  • AGRAWAL, Sudhir; US
Agents
  • GREENFIELD, Michael, S.; Banner & Allegretti, Ltd. Ten South Wacker Drive Chicago, IL 60606, US
Priority Data
08/279,51725.07.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) IMPROVED METHODS OF DETRITYLATION FOR OLIGONUCLEOTIDE SYNTHESIS
(FR) PROCEDES AMELIORES DE DETRITYLATION POUR LA SYNTHESE D'OLIGONUCLEOTIDES
Abstract
(EN)
New methods of synthesizing oligonucleotides are disclosed. The methods result in improved yields by reducing or eliminating depurination that often occurs during detritylation. The method comprises detritylating DMT-blocked oligonucleotides with dichloroacetic acid in combination with a lower alcohol (e.g., methanol or ethanol) or 1H-pyrrole. The method is advantageously used to synthesize oligonucleotides up to about 150 monomers long.
(FR)
Nouveaux procédés de synthèse d'oligonucléotides dont le rendement a été amélioré par réduction ou élimination de la dépurination qui se produit souvent pendant la détritylation et consistant à détrityler les oligonucléotides bloqués par le DMT à l'aide d'acide dichloroacétique combiné à un alcool inférieur (du méthanol ou de l'éthanol p. ex.) ou à du 1H-pyrrole. Le procédé s'avère intéressant pour la synthèse d'oligonucléotides d'une longueur maximale d'environ 150 monomères.
Also published as
Latest bibliographic data on file with the International Bureau