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1. WO1996002672 - METHOD FOR PREVENTING AMPLIFICATION OF NUCLEIC ACID CONTAMINANTS IN AMPLIFICATION MIXTURES

Publication Number WO/1996/002672
Publication Date 01.02.1996
International Application No. PCT/US1995/008782
International Filing Date 18.07.1995
Chapter 2 Demand Filed 15.12.1995
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/6848
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
6848characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Applicants
  • BEHRINGWERKE AKTIENGESELLSCHAFT [DE/DE]; Postfach 11 40 D-35001 Marburg, DE
Inventors
  • ULLMAN, Edwin, F.; US
  • CHEN, Yan; US
  • ROSE, Samuel, J.; US
Agents
  • CLARKE, Pauline, Ann; Building A1-PAT 3401 Hillview Avenue Palo Alto, CA 94304, US
Priority Data
08/277,54719.07.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD FOR PREVENTING AMPLIFICATION OF NUCLEIC ACID CONTAMINANTS IN AMPLIFICATION MIXTURES
(FR) PROCEDE POUR PREVENIR L'AMPLIFICATION DE CONTAMINANTS D'ACIDES NUCLEIQUES DANS DES MELANGES D'AMPLIFICATION
Abstract
(EN)
Methods and kits are disclosed for preventing amplification of contaminating copies of nucleic acids during amplification of a nucleic acid suspected of being present in a sample. Modified nucleotides that render copies of the nucleic acid bindable by a member of a specific binding pair, such as a receptor, which does not bind to the nucleic acid, are incorporated into copies of the nucleic acid that are produced during the amplification. The sample is combined with an enzyme conjugate, usually a receptor bound to a nuclease, under conditions wherein prior to the amplification the member of a specific binding pair binds to the copies and the enzyme degrades the copies but not the nucleic acid. The methods and kits have particular application to the determination of a nucleic acid analyte.
(FR)
Sont décrits des procédés et des trousses pour empêcher l'amplification de copies contaminantes d'acides nucléiques lors de l'amplification d'un acide nucléique soupçonné d'être présent dans un échantillon. Des nucléotides modifiés permettant la fixation de copies de l'acide nucléique par un élément d'une paire fixatrice spécifique, telle qu'un récepteur, lequel ne se fixe pas à l'acide nucléique, sont incorporés dans des copies de l'acide nucléique qui sont produites lors de l'amplification. L'échantillon est associé à un conjugué enzymatique, généralement un récepteur fixé à une nucléase, dans des conditions dans lesquelles, avant l'amplification, l'élément d'une paire fixatrice spécifique se fixe aux copies et l'enzyme dégrade les copies mais pas l'acide nucléique. Ces procédés et trousses s'utilisent notamment pour la détermination d'un analyte d'acide nucléique.
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