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1. WO1996002662 - HUMAN MARROW STROMAL CELL LINES WHICH SUSTAIN HEMATOPOIESIS

Publication Number WO/1996/002662
Publication Date 01.02.1996
International Application No. PCT/US1995/009301
International Filing Date 20.07.1995
Chapter 2 Demand Filed 04.01.1996
IPC
C12N 5/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
02Propagation of single cells or cells in suspension; Maintenance thereof; Culture media therefor
C12N 5/0775 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
077Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
0775Mesenchymal stem cells; Adipose-tissue derived stem cells
C12N 5/0789 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
078Cells from blood or from the immune system
0789Stem cells; Multipotent progenitor cells
CPC
C12N 2501/39
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
30Hormones
38with nuclear receptors
39Steroid hormones
C12N 2502/1394
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2502Coculture with; Conditioned medium produced by
13connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
1394Bone marrow stromal cells; whole marrow
C12N 2510/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2510Genetically modified cells
C12N 2510/04
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2510Genetically modified cells
04Immortalised cells
C12N 5/0647
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0634Cells from the blood or the immune system
0647Haematopoietic stem cells; Uncommitted or multipotent progenitors
Applicants
  • FRED HUTCHINSON CANCER RESEARCH CENTER [US/US]; 1124 Columbia Street Seattle, WA 98104, US (AllExceptUS)
  • TOROK-STORB, Beverly [US/US]; US (UsOnly)
  • ROECKLEIN, Bryan, A. [US/US]; US (UsOnly)
  • JONHSON, Gretchen [US/US]; US (UsOnly)
Inventors
  • TOROK-STORB, Beverly; US
  • ROECKLEIN, Bryan, A.; US
  • JONHSON, Gretchen; US
Agents
  • PARMELEE, Steven, W. ; Townsend and Townsend and Crew Steuart St. Tower 20th floor One Market Plaza San Francisco, CA 94105-1492, US
Priority Data
08/277,88320.07.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) HUMAN MARROW STROMAL CELL LINES WHICH SUSTAIN HEMATOPOIESIS
(FR) LIGNEES CELLULAIRES STROMALES DE LA MOELLE HUMAINE ENTRETENANT L'HEMATOPOIESE
Abstract
(EN)
Immortalized human stromal cell lines sustain and expand human hematopoietic precursor cells. The precursor cells are obtained from a blood product and inoculated into a culture medium conditioned by exposure to a human stromal cell line. Preferred human stromal cell lines secrete SCF, LIF, MIP1$g(a), and IL-6, as exemplified by a human stromal cell line designated HS-1. The conditioned culture medium may be supplemented with additional growth factors, such as SCF and interleukin-3. After expansion the human hematopoietic precursor cells are harvested and returned to a patient or frozen and stored. The immortalized human stromal cell lines can also be used as feeder layers in $i(ex vivo) bone marrow cultures or in colony forming assays.
(FR)
Des lignées cellulaires stromales humaines immortalisées entretiennent et augmentent les cellules précurseurs hématopoïétiques humaines. Ces cellules précurseurs sont extraites d'un produit sanguin et inoculées dans un milieu de culture conditionné par la mise en contact avec une lignée cellulaire stromale humaine. Les lignées cellulaires stromales humaines préférées sécrètent SCF, LIF, MIP1$g(a) et IL-6, selon l'exemple de la lignée cellulaire stromale humaine qu'on appelle HS-1. Le milieu de culture conditionné peut être alimenté en facteurs de croissance supplémentaires, tel que SCF et interleukine-3. Après augmentation, les cellules précurseur hématopoïétiques humaines sont recueillies et réinjectées dans un patient ou congelées et entreposées. Ces lignées cellulaires stromales humaines immortalisées peuvent également être utilisées comme couches d'alimentation dans des culture de moelle osseuse $i(ex vivo) ou dans des analyses de formation de colonies.
Also published as
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