Processing

Please wait...

Settings

Settings

1. WO1996002648 - LKP PILIN STRUCTURAL GENES AND OPERON OF NONTYPABLE HAEMOPHILUS INFLUENZAE

Publication Number WO/1996/002648
Publication Date 01.02.1996
International Application No. PCT/US1995/008789
International Filing Date 13.07.1995
Chapter 2 Demand Filed 13.02.1996
IPC
A61K 39/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
C07K 14/285 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
285from Pasteurellaceae (F), e.g. Haemophilus influenza
C07K 14/47 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
A61K 39/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
C07K 14/285
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
285from Pasteurellaceae (F), e.g. Haemophilus influenza
C07K 14/47
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
C07K 2319/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
C12Q 1/689
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
689for bacteria
Applicants
  • AMERICAN CYANAMID COMPANY [US/US]; One Cyanamid Plaza Wayne, NJ 07470, US (AllExceptUS)
  • BACTEX, INC. [US/US]; 4516 Henry Street Pttsburg, PA 15213, US (AllExceptUS)
  • GREEN, Bruce, A. [US/US]; US (UsOnly)
  • BRINTON, Charles, C., Jr. [US/US]; US (UsOnly)
Inventors
  • GREEN, Bruce, A.; US
  • BRINTON, Charles, C., Jr.; US
Agents
  • CARROLL, Alice, O. ; Hamilton, Brook, Smith & Reynolds Two Militia Drive Lexington, MA 02173, US
Priority Data
08/277,23119.07.1994US
08/473,75007.06.1995US
08/477,32607.06.1995US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) LKP PILIN STRUCTURAL GENES AND OPERON OF NONTYPABLE HAEMOPHILUS INFLUENZAE
(FR) OPERON ET GENES DE STRUCTURE DE PILINE DE LKP DE HAEMOPHILUS INFLUENZAE INCLASSABLES
Abstract
(EN)
The invention relates to the isolation and cloning of the structural gene, $i(hip)P, for the NTHi pili serotype 5 and the LKP operon. The invention relates to DNA molecules capable of hybridizing to the DNA sequences of the $i(Haemophilus influenzae) genome related to the pili. The invention further relates to a DNA molecule which encodes a pili protein, particularly a tip adhesin protein. The DNA molecules of the invention can be used in a method for assaying a sample, such as a blood sample, for the presence of $i(Haemophilus influenzae) in the sample. Accordingly, the invention further relates to the use of the DNA molecules as a diagnostic. The invention also relates to a recombinant $i(Haemophilus influenzae) pili protein, such as a tip adhesin protein. The protein can be employed in a method for immunizing an animal, such as a human, as a therapeutic or diagnostic.
(FR)
L'invention se rapporte à l'isolation et au clonage du gène de structure, $i(hip)P, pour le sérotype 5 des pili de $i(Haemophilus influenzae) inclassables (NTHi) et l'opéron des pili de type long et épais (LKP). L'invention se rapporte également à des molécules d'ADN capables de s'hybrider aux séquences d'ADN du génome de $i(Haemophilus influenzae) associées aux pili, ainsi qu'à une molécule d'ADN qui code une protéine de pili, en particulier une protéine adhésine terminale. Les molécules d'ADN selon l'invention peuvent être utilisées dans un procédé de dosage d'un échantillon, tel qu'un échantillon sanguin, afin d'y détecter la présence d'$i(Haemophilus influenzae). En conséquence, l'invention porte sur l'utilisation de ces molécules d'ADN comme agents diagnostiques, ainsi que sur une protéine de pili d'$i(Haemophilus influenzae) de recombinaison, telle que la protéine adhésine terminale. Cette protéine peut être utilisée dans un procédé permettant d'immuniser un animal et également l'homme, à des fins thérapeutiques et diagnostiques.
Latest bibliographic data on file with the International Bureau