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1. WO1996002633 - NOVEL ACID-STABLE AND THERMO-STABLE ENZYMES DERIVED FROM SULFOLOBUS SPECIES

Publication Number WO/1996/002633
Publication Date 01.02.1996
International Application No. PCT/EP1995/002703
International Filing Date 07.07.1995
Chapter 2 Demand Filed 01.02.1996
IPC
C12N 9/28 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
24acting on glycosyl compounds (3.2)
26acting on alpha-1, 4-glucosidic bonds, e.g. hyaluronidase, invertase, amylase
28Alpha-amylase from microbial source, e.g. bacterial amylase
C12P 19/14 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
14produced by the action of a carbohydrase, e.g. by alpha-amylase
CPC
C12N 9/2417
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
24acting on glycosyl compounds (3.2)
2402hydrolysing O- and S- glycosyl compounds (3.2.1)
2405Glucanases
2408acting on alpha -1,4-glucosidic bonds
2411Amylases
2414Alpha-amylase (3.2.1.1.)
2417from microbiological source
C12P 19/14
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
14produced by the action of a carbohydrase ; (EC 3.2.x); , e.g. by alpha-amylase ; , e.g. by cellulase, hemicellulase
Applicants
  • SOLVAY (SOCIETE ANONYME) [BE/BE]; Rue du Prince-Albert 33 B-1050 Bruxelles, BE (AllExceptUS)
  • DEWEER, Philippe [BE/BE]; BE (UsOnly)
  • AMORY, Antoine [BE/BE]; BE (UsOnly)
Inventors
  • DEWEER, Philippe; BE
  • AMORY, Antoine; BE
Agents
  • MEYERS, Liliane ; Solvay (Société Anonyme) Dept. de la Propriété Industrielle Rue de Ransbeek 310 B-1120 Bruxelles, BE
Priority Data
9414224.714.07.1994GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) NOVEL ACID-STABLE AND THERMO-STABLE ENZYMES DERIVED FROM SULFOLOBUS SPECIES
(FR) NOUVELLES ENZYMES RESISTANTES AUX ACIDES ET A LA CHALEUR, DERIVEES DE L'ESPECE SULFOLOBUS
Abstract
(EN)
Novel acid-stable and thermo-stable enzymes having $g(a)-1,4 hydrolytic activity and a $g(a)-1,6 hydrolytic activity which are derived from strains of the genus $i(Sulfolobus). These enzymes are capable of expressing high levels of $g(a)-1,4 hydrolytic activity, including the maximum $g(a)-1,4 hydrolytic activity thereof, at highly acidic pHs of between about 2.5 and about 4.5. These $g(a)-amylases are further capable of expressing high levels of $g(a)-1,4 hydrolytic activity, including the maximum $g(a)-1,4 hydrolytic activity thereof, at high temperatures of between about 90 °C and about 120 °C. Particularly disclosed herein are such enzymes which are derived from strains of the species $i(S. acidocaldarius) and, in particular, $i(Sulfolobus acidocaldarius) DSM 639. Modified starch degradation (liquefaction and saccharification) processes using these novel enzymes are also disclosed herein.
(FR)
De nouvelles enzymes résistantes aux acides et à la chaleur ayant une activité hydrolytique $g(a)-1,4 et une activité hydrolytique $g(a)-1,6 sont dérivées de souches du genre $i(Sulfolobus). Ces enzymes sont capables d'exprimer de hauts niveaux d'activité hydrolytique $g(a)-1,4, jusqu'à et y compris une activité hydrolytique $g(a)-1,4 maximale, à des valeurs de pH très acides comprises entre 2,5 et 4,5 environ. Ces amylases-$g(a) sont en outre capables d'exprimer de hauts niveaux d'activité hydrolytique $g(a)-1,4, jusqu'à et y compris une activité hydrolytique $g(a)-1,4 maximale, à de hautes températures comprises entre 90 °C et 120 °C environ. L'invention concerne notamment les enzymes dérivées de souches des espèces $i(S. Acidocaldarius), tout particulièrement $i(Sulfolobus acidocaldarius) DSM 639. L'invention concerne en outre des processus modifiés de dégradation d'amidon (liquéfaction et saccharification) au moyen de ces enzymes.
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