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1. WO1996002627 - DIFFUSION CHAMBER SYSTEM AND METHOD FOR TRANSPORT STUDIES

Publication Number WO/1996/002627
Publication Date 01.02.1996
International Application No. PCT/US1995/008524
International Filing Date 13.07.1995
Chapter 2 Demand Filed 12.02.1996
IPC
C12M 1/42 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
42Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
C12M 3/06 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
06with filtration, ultrafiltration, inverse osmosis or dialysis means
G01N 13/00 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
13Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
CPC
C12M 25/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
25Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
02Membranes; Filters
04in combination with well or multiwell plates, i.e. culture inserts
G01N 13/00
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
13Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
Applicants
  • SMITHKLINE BEECHAM CORPORATION [US]/[US] (AllExceptUS)
  • HIDALGO, Ismael, Juvenal [PA]/[US] (UsOnly)
  • RYAN, Frederick, Michael [US]/[US] (UsOnly)
  • SMITH, Philip, Lawrence [US]/[US] (UsOnly)
Inventors
  • HIDALGO, Ismael, Juvenal
  • RYAN, Frederick, Michael
  • SMITH, Philip, Lawrence
Agents
  • SUTTON, Jeffrey, A.
Priority Data
08/274,88314.07.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) DIFFUSION CHAMBER SYSTEM AND METHOD FOR TRANSPORT STUDIES
(FR) SYSTEME DE CHAMBRE DE DIFFUSION ET PROCEDE POUR REALISER DES ETUDES DE TRANSPORT
Abstract
(EN)
A diffusion chamber system including in combination an Ussing diffusion chamber (10) and fluid circulating device (18) for transport studies using membranous barriers such as excised animal tissue cultered cell monolayers. The chamber is provided with recesses (32, 34) in two mating chamber halves (20, 22) to receive a barrier support on an insert (36). Potential electrodes (114, 116) extend obliquely through the walls of the chamber halves (20, 22) and into both ends of the insert (36) so that the tips (128, 130) of the electrodes (114, 116) are in close proximity to, and centered on, the barrier.
(FR)
Un système de chambre de diffusion comprend en combinaison une chambre de diffusion d'Ussing (10) et un dispositif (18) de circulation de fluide, pour effectuer des études de transport utilisant des barrières membranaires telles que des monocouches de cellules de culture provenant de tissus d'animaux excisés. La chambre comporte des creux (32, 34) dans deux moitiés (20, 22) de chambre s'adaptant l'une à l'autre et recevant un support de barrière sur un élément d'insertion (36). Des électrodes à potentiel (114, 116) traversent obliquement les parois des moitiés (20, 22) de chambre et pénètrent dans les deux extrémités de l'élément d'insertion (36) de manière que les extrémités (128, 130) des électrodes (114, 116) soient proches de la barrière et centrées par rapport à celle-ci.
Also published as
Latest bibliographic data on file with the International Bureau