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1. WO1996002563 - EPSTEIN-BARR VIRUS NUCLEAR ANTIGEN 1 PROTEIN AND ITS EXPRESSION AND RECOVERY

Publication Number WO/1996/002563
Publication Date 01.02.1996
International Application No. PCT/US1995/008700
International Filing Date 13.07.1995
Chapter 2 Demand Filed 09.02.1996
IPC
C07K 14/05 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
01DNA viruses
03Herpetoviridae, e.g. pseudorabies virus
05Epstein-Barr virus
CPC
C07K 14/005
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
C12N 2710/14143
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
14011Baculoviridae
14111Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
14141Use of virus, viral particle or viral elements as a vector
14143viral genome or elements thereof as genetic vector
C12N 2710/16222
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
16011Herpesviridae
16211Lymphocryptovirus, e.g. human herpesvirus 4, Epstein-Barr Virus
16222New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Applicants
  • CORNELL RESEARCH FOUNDATION, INC. [US/US]; Suite 105 20 Thornwood Drive Ithaca, NY 14850, US
Inventors
  • O'DONNELL, Michael, E.; US
Agents
  • GOLDMAN, Michael, L. ; Nixon, Hargrave, Devans & Doyle Clinton Square P.O. Box 1051 Rochester, NY 14603, US
Priority Data
08/275,61413.07.1994US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) EPSTEIN-BARR VIRUS NUCLEAR ANTIGEN 1 PROTEIN AND ITS EXPRESSION AND RECOVERY
(FR) PROTEINE DE L'ANTIGENE NUCLEAIRE 1 DU VIRUS EPSTEIN BARR, SON EXPRESSION ET SA RECUPERATION
Abstract
(EN)
A process for expressing and recovering Epstein-Barr nuclear antigen 1 (EBNA1) protein or polypeptide treats cells having a nucleus containing expressed EBNA1 protein or polypeptide to recover the nucleus containing the expressed EBNA1 protein or polypeptide. The nucleus containing the expressed EBNA1 protein or polypeptide is then separated into a liquid fraction containing the expressed EBNA1 protein or polypeptide and a solid fraction containing substantially all DNA from the nucleus. The liquid fraction is separated from the solid fraction, and EBNA1 protein or polypeptide is recovered from the liquid fraction. Also encompassed by the present invention is an EBNA1 protein or polypeptide having substantially no components which generate false positive readings when used to detect Epstein-Barr virus in human serum, the DNA molecule encoding it, and recombinant expression of the protein. The protein is useful in a method for detection of Epstein-Barr virus.
(FR)
L'invention se rapporte à un procédé d'expression et de récupération d'une protéine ou d'un polypeptide (EBNA1) de l'antigène nucléaire 1 du virus Epstein Barr consistant à traiter des cellules dont le noyau contient la protéine ou le polypeptide EBNA1 exprimé afin de récupérer le noyau contenant la protéine ou le polypeptide EBNA1 exprimé. Le noyau contenant la protéine ou le polypeptide EBNA1 exprimé est ensuite séparé dans une fraction liquide contenant la protéine ou le polypeptide EBNA1 exprimé et dans une fraction liquide contenant pratiquement tout l'ADN provenant du noyau. La fraction liquide est séparée de la fraction solide, et la protéine ou le polypeptide EBNA1 est récupéré à partir de la fraction liquide. L'invention se rapporte également à une protéine ou un polypeptide EBNA1 ne possédant pas de composants générant des lectures faux positif lorsqu'ils sont utilisés pour détecter le virus Epstein Barr dans le sérum humain, à la molécule d'ADN codant cette protéine et à l'expression par recombinaison de cette protéine. Cette protéine est utilisée dans un procédé de détection du virus Epstein Barr.
Also published as
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