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1. (WO1994028421) INHIBITORS OF NEF-CD4 BINDING

Pub. No.:    WO/1994/028421    International Application No.:    PCT/GB1994/001172
Publication Date: Dec 8, 1994 International Filing Date: May 31, 1994
IPC: G01N 33/569
Applicants: MEDICAL RESEARCH COUNCIL
THE UNIVERSITY COURT OF THE UNIVERSITY OF GLASGOW
HARRIS, Mark, Peter, Gerard
NEIL, James, Charles
Inventors: HARRIS, Mark, Peter, Gerard
NEIL, James, Charles
Title: INHIBITORS OF NEF-CD4 BINDING
Abstract:
An assay to determine the inhibitory effect of a substance on the interaction of Nef protein with a CD4 cell surface marker comprises a source of myristoylated Nef protein and at least the cytoplasmic domain of CD4 glycoprotein. The myristoylated Nef and CD4 glycoprotein may be co-expressed in a cell which has been co-infected with recombinant viruses which express them. The cells are cultured in the presence of a potentially inhibitory substance and the inhibitory effect on the production of myristoylated Nef-CD4 complex determined.