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1. (WO1994016105) RNA ASSAYS USING RNA BINARY PROBES AND RIBOZYME LIGASE
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/1994/016105 International Application No.: PCT/US1994/000470
Publication Date: 21.07.1994 International Filing Date: 14.01.1994
Chapter 2 Demand Filed: 15.08.1994
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
THE PUBLIC HEALTH RESEARCH INSTITUTE OF THE CITY OF NEW YORK, INC. [US/US]; 455 First Avenue New York, NY 10016, US
THE GENERAL HOSPITAL CORPORATION [US/US]; Fruit Street Boston, MA 02114, US
Inventors:
LIZARDI, Paul, M.; MX
TYAGI, Sanjay; US
LANDEGREN, Ulf, D.; SE
KRAMER, Fred, R.; US
SZOSTAK, Jack, W.; US
Agent:
JACOBS, Seth, H. ; Davis Hoxie Faithfull & Hapgood 45 Rockefeller Plaza New York, NY 10111, US
Priority Data:
08/005,89315.01.1993US
Title (EN) RNA ASSAYS USING RNA BINARY PROBES AND RIBOZYME LIGASE
(FR) DOSAGES D'ARN AU MOYEN DE SONDES BINAIRES D'ARN ET D'UNE RIBOZYME-LIGASE
Abstract:
(EN) There are provided nucleic acid hybridization assays for RNA targets using RNA binary probes and a ribozyme ligase that is a stringent RNA-directed RNA ligase. Preferred assays include exponential amplification for signal generation. $i(Tetrahymena) ribozyme ligase is a preferred ligase for use in this invention. It may be tethered to hold it close to the ligation junction. One assay according to this invention is a ''tethered ligase chain reaction''. Also provided are kits for performing assays according to the invention.
(FR) L'invention se rapporte à des dosages par hybridation d'acide nucléique, destinés à des ARN cibles et consistant à utiliser des sondes binaires d'ARN, ainsi qu'une ribozyme-ligase qui est une ARN-ligase ARN-dépendante stringente. Des dosages préférés comprennent l'amplification exponentielle permettant la génération de signaux. La ribozyme-ligase de $i(Tetrahymena) est de préférence utilisée. Elle peut être fixée afin d'être retenue à proximité de la jonction de ligature. Selon cette invention, un dosage utilisé est une ''amplification par ligase captive''. Des kits permettant d'effectuer les dosages décrits sont également décrits.
Designated States: AU, CA, JP
European Patent Office (EPO) (AT, BE, CH, DE, DK, ES, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: English (EN)
Filing Language: English (EN)