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1. (WO1994014970) METHOD FOR OBTAINING TRANSGENIC PLANTS SHOWING A MODIFIED FRUCTAN PATTERN

Pub. No.:    WO/1994/014970    International Application No.:    PCT/NL1993/000279
Publication Date: Jul 7, 1994 International Filing Date: Dec 28, 1993
IPC: A23K 1/14
A23L 1/0528
C12N 9/10
C12N 15/82
Applicants: STICHTING SCHEIKUNDIG ONDERZOEK IN NEDERLAND
SMEEKENS, Josephus, Christianus, Maria
EBSKAMP, Michaël, Johannes, Marcus
WEISBEEK, Petrus, Jacobus
Inventors: SMEEKENS, Josephus, Christianus, Maria
EBSKAMP, Michaël, Johannes, Marcus
WEISBEEK, Petrus, Jacobus
Title: METHOD FOR OBTAINING TRANSGENIC PLANTS SHOWING A MODIFIED FRUCTAN PATTERN
Abstract:
The invention relates to a method for obtaining transgenic plants showing a modified fructan pattern as compared to non-transformed plants, comprising the steps of: a) preparing a DNA construct comprising one or more fructosyltransferase genes, or modified versions thereof, being operably linked to a promoter sequence active in plants and a terminator sequence active in plants; b) transforming a plant cell with the construct; and c) regenerating a transgenic plant from the transformed plant cell. Preferably, the 5' untranslated region of the fructosyltransferase gene is modified such that the expression of the fructosyltransferase is not negatively affected. The DNA construct preferably also comprises a targeting sequence upstream from the fructosyltransferase gene for directing the fructosyltransferase to a specific plant tissue or plant cell compartment. The fructosyltransferases produced in the transgenic plants change the fructan pattern of the plants thus leading to different plant performance due to altered sink-source relations and yield, or increased tolerance for drought, cold or other stresses, higher dry matter content, better taste or storability, improved nutritional value. The plants may also suitably be used as raw material for fructan production.