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1. (WO1994003624) METHODS FOR THE ISOTHERMAL AMPLIFICATION OF NUCLEIC ACID MOLECULES
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/1994/003624 International Application No.: PCT/US1993/007309
Publication Date: 17.02.1994 International Filing Date: 04.08.1993
Chapter 2 Demand Filed: 15.10.1993
IPC:
C12N 15/10 (2006.01) ,C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
AUERBACH, Jeffrey, I. [US/US]; US
Inventors:
AUERBACH, Jeffrey, I.; US
Priority Data:
07/924,64304.08.1992US
07/933,94524.08.1992US
Title (EN) METHODS FOR THE ISOTHERMAL AMPLIFICATION OF NUCLEIC ACID MOLECULES
(FR) PROCEDES D'AMPLIFICATION ISOTHERMIQUE DE MOLECULES D'ACIDE NUCLEIQUE
Abstract:
(EN) Methods for amplifying a nucleic acid molecule which employs a single primer, and in which the amplification is performed under isothermal conditions. The invention also includes kits containing reagents for conducting the method. The figure shows the twin origin 'rolling circle' replicon that results from the extension of two primers during the amplification of a single-stranded circular molecule.
(FR) Procédés d'amplification d'une molécule d'acide nucléique consistant à utiliser une amorce unique, et selon lesquels l'amplification est effectuée dans des conditions isothermiques. L'invention se rapporte également à des nécessaires contenant des réactifs permettant d'appliquer ce procédé. La figure 8A illustre le réplicon à 'cercle tournant' et à double origine produit par le prolongement de deux amorces au cours de l'amplification d'une molécule circulaire monocaténaire.
Designated States: CA, JP, US
European Patent Office (EPO) (AT, BE, CH, DE, DK, ES, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: English (EN)
Filing Language: English (EN)