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Machine translation
1. (WO1993021303) ENHANCED PURIFICATION AND EXPRESSION OF INSOLUBLE RECOMBINANT PROTEINS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1993/021303    International Application No.:    PCT/US1993/003652
Publication Date: 28.10.1993 International Filing Date: 16.04.1993
Chapter 2 Demand Filed:    16.11.1993    
IPC:
A61K 48/00 (2006.01), C07K 1/113 (2006.01), C07K 14/155 (2006.01), C07K 14/18 (2006.01), C07K 14/53 (2006.01), C07K 14/55 (2006.01), C07K 14/565 (2006.01), C12N 9/02 (2006.01), C12N 15/85 (2006.01), C12N 15/88 (2006.01), C12N 15/90 (2006.01), C12P 21/02 (2006.01)
Applicants: CHIRON CORPORATION [US/US]; 4560 Horton Street, Emeryville, CA 94608 (US)
Inventors: COUSENS, Lawrence, S.; (US).
TEKAMP-OLSON, Patricia; (US)
Agent: CHUNG, Ling-Fong; Chiron Corporation, Intellectual Property Department, 4560 Horton Street, Emeryville, CA 94608 (US)
Priority Data:
07/869,613 16.04.1992 US
Title (EN) ENHANCED PURIFICATION AND EXPRESSION OF INSOLUBLE RECOMBINANT PROTEINS
(FR) PURIFICATION ET EXPRESSION AMELIOREES DE PROTEINES RECOMBINEES INSOLUBLES
Abstract: front page image
(EN)A method for isolating a desired protein which comprises: providing a host cell expressing the desired protein as an insoluble aggregate; culturing the host cell with an effective amount of Cu?++¿; disrupting the host cell producing a lysate; incubating the insoluble fraction under non-disulfide-bond reducing or non-copper competing chaotropic conditions to solubilize contaminants; separating the insoluble from the soluble fraction; and exposing the insoluble fraction to disulfide-bond reducing or copper competing chaotropic conditions to solubilize the desired protein.
(FR)Un procédé d'isolement d'une protéine voulue consiste à produire une cellule hôte exprimant la protéine voulue sous la forme d'un agrégat insoluble, à mettre en culture la cellule hôte avec une dose efficace de Cu?++¿, à désunir la cellule hôte pour produire un lysat, à mettre en incubation la fraction insoluble dans des conditions chaotropes non réductrices de ponts disulfure ou non concurrentes du cuivre afin de solubiliser les contaminants, à séparer la fraction insoluble de la fraction soluble, et à exposer la fraction insoluble à des conditions chaotropes réductrices de ponts disulfure ou concurrentes du cuivre afin de solubiliser la protéine voulue.
Designated States: CA, JP.
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE).
Publication Language: English (EN)
Filing Language: English (EN)