WO1993005163 METHOD FOR THE SELECTION OF GENETICALLY TRANSFORMED CELLS AND COMPOUNDS FOR USE IN THE METHOD

Settings

Stemming

Single Family Member

Include NPL

Feedback

Goto Application

Publication Number WO/1993/005163

Publication Date 18.03.1993

International Application No. PCT/DK1992/000252

International Filing Date 27.08.1992

Chapter 2 Demand Filed 24.09.1992

IPC

View more classifications

View less classifications

CPC

View more classifications

View less classifications

Applicants

SANDOZ LTD. (AllExceptUS)
OKKELS, Finn, T. [DK]/[DK] (UsOnly)
WHENHAM, Robert, J. [GB]/[GB] (UsOnly)

Inventors

OKKELS, Finn, T.
WHENHAM, Robert, J.

Common Representative

SANDOZ LTD.

Priority Data

1522/91

28.08.1991

Publication Language English (EN)

Filing Language English (EN)

Designated States

View all

Title

(EN) METHOD FOR THE SELECTION OF GENETICALLY TRANSFORMED CELLS AND COMPOUNDS FOR USE IN THE METHOD

(FR) PROCEDE DE SELECTION DE CELLULES TRANSFORMEES GENETIQUEMENT ET COMPOSES DESTINES A ETRE UTILISES DANS LEDIT PROCEDE

Abstract

(EN)

A method for selecting from a population of cells genetically transformed cells into which a desired nucleotide sequence has been introduced, wherein in the transformed cells the desired nucleotide sequence or a co-introduced nucleotide sequence induces or increases a positive effect of a compound or nutrient supplied to the population of cells, thereby allowing the transformed cells to be identified or selected from non-transformed cells, e.g. for the preparation of genetically transformed plants not containing as a selection marker a non-native nucleotide sequence coding for toxing, antibiotic or herbicide resistance; as well as novel glucuronide compounds, including cytokinin glucuronide compounds, for use in the method.

(FR)

Procédé de sélection de cellules, à partir d'une population de cellules génétiquement transformées, dans lesquelles on a introduit une séquence nucléotide désirée. Selon le procédé, dans lesdites cellules transformées, la séquence nucléotide désirée ou une séquence nucléotide introduite conjointement induit ou augmente l'effet positif d'un composé ou d'une substance nutritive fournie à la population de cellules, permettant ainsi l'identification ou la sélection des cellules transformées à partir de cellules non-transformées, par exemple, pour la préparation de plantes génétiquement transformées ne contenant pas comme marqueur de sélection une séquence nucléotide non-indigène codant pour une résistance aux toxines, aux antibiotiques ou aux herbicides. L'invention concerne également des composés de glucuronide comprenant les composés de glucuronide cytokines destinés à être utilisés dans le procédé.

Also published as

DK152291

ES2135415

EP0530129

EP0896063

DE000069229556

PT601092

EP0601092

RU02126834

AU1992025567

DK0601092

JP1994511146

SK19940222

CZ284828

CZ292882

CA2110401

US5994629

GR3031279

Latest bibliographic data on file with the International Bureau

A	HUMAN NECESSITIES
01	AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H	NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5	Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
12	Leaves

C	CHEMISTRY; METALLURGY
07	ORGANIC CHEMISTRY
H	SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
15	Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
20	Carbocyclic rings
203	Monocyclic carbocyclic rings other than cyclohexane rings; Bicyclic carbocyclic ring systems

C	CHEMISTRY; METALLURGY
07	ORGANIC CHEMISTRY
H	SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
15	Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
26	Acyclic or carbocyclic radicals, substituted by hetero rings

C	CHEMISTRY; METALLURGY
07	ORGANIC CHEMISTRY
H	SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
17	Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
02	Heterocyclic radicals containing only nitrogen as ring hetero atoms

C	CHEMISTRY; METALLURGY
07	ORGANIC CHEMISTRY
H	SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
19	Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro derivatives thereof
02	sharing nitrogen
04	Heterocyclic radicals containing only nitrogen as ring hetero atom
16	Purine radicals

Processing

National Phase Entries

Authority File

Settings

Feedback

Goto Application

1. WO1993005163 - METHOD FOR THE SELECTION OF GENETICALLY TRANSFORMED CELLS AND COMPOUNDS FOR USE IN THE METHOD

WIPO Translate

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5	Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor

A	HUMAN NECESSITIES
01	AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H	NEW PLANTS OR ; NON-TRANSGENIC; PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5	Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
12	Leaves

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Y	ENZYMES
302	Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
01	Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
01031	Beta-glucuronidase (3.2.1.31)

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
65	using markers

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79	Vectors or expression systems specially adapted for eukaryotic hosts
82	for plant cells

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
65	using markers

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79	Vectors or expression systems specially adapted for eukaryotic hosts
82	for plant cells ; , e.g. plant artificial chromosomes (PACs)
8201	Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
8209	Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers