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1. WO1992017598 - PRODUCTION FO TRANSGENIC SOYBEAN PLANTS

Publication Number WO/1992/017598
Publication Date 15.10.1992
International Application No. PCT/US1992/002226
International Filing Date 26.03.1992
IPC
A01H 4/00 2006.1
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
4Plant reproduction by tissue culture techniques
A01H 5/10 2006.1
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
10Seeds
C12N 15/82 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
CPC
A01H 4/00
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR ; NON-TRANSGENIC; PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
4Plant reproduction by tissue culture techniques ; ; Tissue culture techniques therefor
A01H 5/10
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR ; NON-TRANSGENIC; PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
10Seeds
C12N 15/8206
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
8206by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
Applicants
  • THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS [US]/[US] (AllExceptUS)
  • WIDHOLM, Jack, M. [US]/[US] (UsOnly)
  • DHIR, Sarwan, K. [IN]/[US] (UsOnly)
Inventors
  • WIDHOLM, Jack, M.
  • DHIR, Sarwan, K.
Agents
  • DELUCA, Mark
Priority Data
677,39829.03.1991US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) PRODUCTION FO TRANSGENIC SOYBEAN PLANTS
(FR) PRODUCTION D'ESPECES DE SOJA TRANSGENIQUES
Abstract
(EN) A method of generating transgenic soybean plants comprising the steps of: preparing protoplasts from soybean cotyledons; inserting foreign DNA into the prepared protoplasts by electroporation, the foreign DNA comprising a useful gene; culturing the electroplated protoplasts in medium to induce cell growth, colony formation and calli generation; and regenerating plants from calli is disclosed.
(FR) Un procédé de production d'espèces de soja transgéniques comprend les étapes suivantes: préparation de protoplastes à partir de cotylédons de soja; insertion par électroporation d'un ADN étranger dans les protoplastes préparés, ledit ADN comprenant un gène utile; culture des protoplastes électroplaqués dans un milieu pour induire la croissance des cellules, la formation de colonies et la génération de cal; et regénération de plantes à partir dudit cal.
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